Immunofluorescent analysis of Galectin 1 showing staining in the cytoplasm of A549 cells. A549 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Galectin 1 polyclonal antibody (Product # PA5-27455) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 135 of Human Galectin 1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27455 targets Galectin 1 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-27455 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 135 of Human Galectin 1.
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.