Invitrogen

Galectin 1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (37) Galectin 1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Galectin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Galectin 1 was performed using 70% confluent log phase MDA-MB-231 and MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Galectin 1 Polyclonal Antibody (Product # PA5-30706) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization of Galectin 1 in MDA-MB-231. Panel e represents MCF7 cells showing negative staining for the same. Panel f represents control MDA-MB-231 cells with no primary antibody to assess background. The images were captured at 60X magnification.

Galectin 1 Antibody in Western Blot (WB) — Western blot was performed using Anti-Galectin 1 Polyclonal Antibody (Product # PA5-30706) and a 15kDa band corresponding to Galectin 1 was observed in MDA-MB-231, THP-1, PC-3 and Caki-1 but not in MCF7, LNCaP. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MCF7 (Lane 2), THP-1 (Lane 3), LNCaP (Lane 4), PC-3 (Lane 5) and Caki-1 (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:15000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Increased expression of Galectin-1 is seen in triple negative breast cancer (TNCB) (like MDA-MB-231) for promoting metastasis but not in non-TNBC like MCF7 [10.18632/oncotarget.16208] and in androgen unresponsive PC-3 cells in comparison to androgen responsive LNCaP [10.1155/2013/519436].

Galectin 1 Antibody in Immunoprecipitation (IP) — Galectin 1 Polyclonal Antibody immunoprecipitates Galectin 1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract. A : 30 µg whole cell lysate/extract of Galectin 1 Polyclonal Antibody expressing HeLa cells. B : Control with 2.5 µg of pre-immune rabbit IgG. C : Immunoprecipitation of Galectin 1 by 2.5 µg of Galectin 1 Polyclonal Antibody (Product # PA5-30706). 15% SDS-PAGE. The immunoprecipitated Galectin 1 protein was detected by Galectin 1 Polyclonal Antibody (Product # PA5-30706) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.

Galectin 1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. PC-3 cells were transfected with Galectin 1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-Galectin 1 Polyclonal Antibody (Product # PA5-30706).

Product Details

PA5-30706

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Guinea pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length human Galectin 1 Recombinant protein.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2548180

Product Specific Information

Recommended positive controls: K562, THP-1, HL-60, 293T, NCI-H929, BCL-1, PC-12, Rat2.

Predicted reactivity: Mouse (88%), Rat (90%), Dog (82%), Pig (85%), Sheep (85%), Rhesus Monkey (97%), Bovine (86%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantitative proteomic analysis of the brainstem following lethal sarin exposure.

Brain research

Meade ML,Hoffmann A,Makley MK,Snider TH,Schlager JJ,Gearhart JM

PA5-30706 was used in western blot to study the effects of sarin on the guinea pig brainstem.

Mon Jun 22 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: 14 kDa laminin-binding protein; 14 kDa lectin; beta-galactoside binding protein; Beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa; DKFZp686E23103; Gal-1; Galaptin; Galectin-1; Galectin1; H-Galectin-1; HBL; HLBP14; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactose binding, soluble 1; lectin, galactoside binding soluble 1; lectin, galactoside-binding, soluble, 1; pm12; Putative MAPK-activating protein PM12; RL 14.5; S-Lac lectin 1

Gene Aliases: AA410090; Gal-1; GAL1; Galbp; galectin-1; GBP; L-14.5; L14; Lect14; LGALS1

UniProt ID: (Human) P09382, (Mouse) P16045, (Rat) P11762

Entrez Gene ID: (Human) 3956, (Mouse) 16852, (Rat) 56646

Molecular Function: extracellular matrix protein