Immunofluorescent analysis of Galectin 3 showing staining in the cytoplasm of MCF-7 cells. MCF-7 cells were fixed in ice-cold MeOH for 5 min and stained using a Galectin 3 polyclonal antibody (Product # PA5-34819) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 184 of Galectin3 (Uniprot ID#P17931)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34819 targets Galectin 3 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-34819 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 184 of Galectin3 (Uniprot ID#P17931).
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis . In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.