Western blot analysis of Galectin 7 using 30µg of A) Non-transfected 293T lysates and B) LGALS7 transfected 293T lysates lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a Galectin 7 polyclonal antibody (Product # PA5-31866) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 86 of Human Galectin 7|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31866 targets Galectin 7 in WB applications and shows reactivity with Human samples.
The PA5-31866 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 86 of Human Galectin 7.
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridization studies indicate that this lectin is specifically expressed in keratinocytes and found mainly in stratified squamous epithelium. A duplicate copy of this gene (GeneID:653499) is found adjacent to, but on the opposite strand on chromosome 19.
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