Western blot analysis of Galectin 8 using 30µg of A) Non-transfected 293T lysates and B) LGALS8 transfected 293T lysates lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Galectin 8 polyclonal antibody (Product # PA5-28506) at a dilution of 1:5000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 108 and 317 of Human Galectin 8|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28506 targets Galectin 8 in WB applications and shows reactivity with Human samples.
The PA5-28506 immunogen is recombinant fragment corresponding to a region within amino acids 108 and 317 of Human Galectin 8.
This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified.
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