Western blot analysis of Galectin 9 using 30µg of A) Non-transfected 293T lysates and B) LGALS9 transfected 293T lysates lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Galectin 9 polyclonal antibody (Product # PA5-29823) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 142 and 355 of Human Galectin 9|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29823 targets Galectin 9 in WB applications and shows reactivity with Human samples.
The PA5-29823 immunogen is recombinant fragment corresponding to a region within amino acids 142 and 355 of Human Galectin 9.
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene.
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