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Invitrogen

Gata-4 Monoclonal Antibody (eBioEvan), eBioscience™

Advanced Verification
4 Published Figures
16 References
View all (30) GATA4 antibodies

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Product Details

14-9980-82

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

-
View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

-
View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 8 publications 8 publications
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

eBioEvan

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_763541

Product Specific Information

Description: The eBioEvan monoclonal antibody reacts with mouse and human Gata-4. In a western blot of C57Bl/6 and BALB/c mouse heart lysates, a single band is present, suggesting that this antibody does not cross-react with Gata-5 or Gata-6, which are also expressed in the heart, but with different molecular weights. The eBioEvan monoclonal antibody has been successfully used in flow cytometry on HEK 293T cells transiently transfected with either mouse Gata-4 or human Gata-4.

Gata-4 is a member of the GATA family of transcription factors, which are characterized by a conserved DNA binding domain containing two zinc fingers. The mouse Gata-4 gene encodes a protein of 48 kDa. Studies show that Gata-4 plays an important role in controlling cardiomyocyte differentiation, proliferation and survival. Gata-4 is expressed during fetal development, as well as in the adult mouse heart. It also appears to modulate gene expression in the gut, liver, and gonads.

Applications Reported: This eBioEvan antibody has been reported for use in immunoblotting (WB) and immunohistochemistry.

Applications Tested: This eBioEvan antibody has been tested by western blot analysis of mouse heart lysates. As a starting dilution, it is recommended to use this purified antibody at 2 µg/mL for western blot analysis. The eBioEvan antibody has been tested on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: GAT4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; putative; Transcription factor GATA-4; unnamed protein product

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Gene Aliases: ASD2; Gata-4; GATA4; TACHD; TOF; VSD1

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UniProt ID: (Human) P43694, (Mouse) Q08369

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Entrez Gene ID: (Human) 2626, (Mouse) 14463

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding protein kinase binding sequence-specific DNA binding metal ion binding NFAT protein binding RNA polymerase II sequence-specific DNA binding transcription factor binding co-SMAD binding DNA-binding transcription factor binding sequence-specific double-stranded DNA binding transcription factor activity, RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding chromatin binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity transcription factor binding activating transcription factor binding transcription regulatory region DNA binding DNA-binding transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter heart looping atrioventricular node development aortic valve morphogenesis atrioventricular valve formation endocardial cushion development cardiac ventricle morphogenesis cardiac right ventricle morphogenesis ventricular septum development atrial septum primum morphogenesis atrial septum secundum morphogenesis regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cell-cell signaling endoderm development heart development male gonad development response to xenobiotic stimulus response to mechanical stimulus anatomical structure morphogenesis tissue development negative regulation of autophagy positive regulation of vascular endothelial growth factor production negative regulation of cardiac muscle cell apoptotic process cell differentiation positive regulation of BMP signaling pathway response to vitamin A embryonic heart tube anterior/posterior pattern specification atrioventricular canal development wound healing cell fate commitment positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic foregut morphogenesis cardiac muscle cell differentiation transdifferentiation atrial septum morphogenesis intestinal epithelial cell differentiation cardiac muscle tissue regeneration cell growth involved in cardiac muscle cell development cellular response to glucose stimulus regulation of cardiac muscle cell contraction negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of apoptotic signaling pathway in utero embryonic development gastrulation with mouth forming second endoderm formation heart morphogenesis outflow tract morphogenesis atrioventricular valve morphogenesis mitral valve formation tricuspid valve formation ventricular cardiac muscle tissue development cardiac septum development transcription, DNA-templated transcription from RNA polymerase II promoter spermatogenesis regulation of gene expression cardiac muscle hypertrophy in response to stress signal transduction involved in regulation of gene expression BMP signaling pathway response to retinoic acid embryonic heart tube development tube morphogenesis skeletal muscle cell differentiation response to drug response to estrogen embryonic digestive tract morphogenesis embryonic morphogenesis cardiac muscle tissue development Sertoli cell differentiation regulation of cardiac muscle cell proliferation positive regulation of cardiac muscle cell proliferation canonical Wnt signaling pathway SMAD protein signal transduction lung morphogenesis lung lobe formation diaphragm morphogenesis vasculogenesis involved in coronary vascular morphogenesis cellular response to gonadotropin stimulus cellular response to follicle-stimulating hormone stimulus epithelial cell fate commitment seminiferous tubule development
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