Invitrogen

Gelsolin Polyclonal Antibody

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Gelsolin Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Gelsolin in NIH3T3 cells. Sample was incubated with Gelsolin polyclonal antibody (Product # PA5-79332).

Gelsolin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Gelsolin on paraffin-embedded human intestinal cancer tissue. Sample was incubated with Gelsolin polyclonal antibody (Product# PA5-79332).

Gelsolin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Gelsolin on paraffin-embedded rat cardiac muscle tissue. Sample was incubated with Gelsolin polyclonal antibody (Product# PA5-79332).

Gelsolin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Gelsolin on paraffin-embedded human lung cancer tissue. Sample was incubated with Gelsolin polyclonal antibody (Product# PA5-79332).

Gelsolin Antibody in Western Blot (WB) — Western blot analysis of Gelsolin in Lane 1: HeLa whole cell lysate, Lane 2: A431 whole cell lysate using 40-50 µg per well. Sample was incubated with Gelsolin (Product # PA5-79332) at a dilution of 0.5 µg/mL.

Product Details

PA5-79332

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-0.5 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	0.5-1 µg/mL	-
Immunocytochemistry (ICC/IF)	2 µg/mL	-
Flow Cytometry (Flow)	1-3 µg/1x10^6 cells	-

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human Gelsolin (763-775aa WDDDYWSVDPLDR).
Conjugate	Unconjugated
Form	Lyophilized
Concentration	500 µg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5mg BSA
Contains	0.05mg sodium azide, 0.05mg thimerosal
Storage conditions	-20°C
RRID	AB_2746448

Product Specific Information

Reconstitute with 0.2 ml of distilled water to yield a concentration of 500 µg/ml.

Target Information

The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Actin-depolymerizing factor; ADF; ADFBrevin; AGEL; Brevin; DKFZp313L0718; Gelsolin; RP11-477J21.1

Gene Aliases: ADF; AGEL; Gsb; GSN

UniProt ID: (Human) P06396, (Rat) Q68FP1, (Mouse) P13020

Entrez Gene ID: (Human) 2934, (Rat) 296654, (Mouse) 227753

Molecular Function: non-motor actin binding protein

phagocytosis, engulfment aging positive regulation of gene expression oligodendrocyte development striated muscle atrophy extracellular matrix disassembly actin filament polymerization protein destabilization tissue regeneration sequestering of actin monomers cellular protein metabolic process actin nucleation response to ethanol negative regulation of viral entry into host cell phosphatidylinositol-mediated signaling actin filament severing barbed-end actin filament capping positive regulation of actin nucleation response to folic acid actin filament capping cilium morphogenesis cellular response to cadmium ion regulation of podosome assembly actin filament reorganization renal protein absorption hepatocyte apoptotic process positive regulation of keratinocyte apoptotic process regulation of wound healing, spreading of epidermal cells regulation of establishment of T cell polarity regulation of plasma membrane raft polarization regulation of receptor clustering positive regulation of protein processing in phagocytic vesicle amyloid fibril formation positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway apoptotic process actin polymerization or depolymerization vesicle-mediated transport regulation of cell adhesion wound healing cell projection organization

Process(es)

actin binding calcium ion binding protein binding protein domain specific binding myosin II binding metal ion binding