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Gelsolin Antibody (PA5-79332) in ICC/IF
Immunocytochemistry analysis of Gelsolin using anti-Gelsolin antibody (Product # PA5-79332). Gelsolin was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Gelsolin antibody (Product # PA5-79332) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained w... View More
Product Details
PA5-79332
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Gelsolin (763-775aa WDDDYWSVDPLDR).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746448
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human THP-1 whole cell, human U251 whole cell, monkey COS-7 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell. IHC: Human Lung Cancer tissue, Human Intestinal Cancer tissue, Rat Cardiac Muscle tissue. ICC/IF: U20S cell, NIH3T3 Cell. Flow: U87 cell.
Target Information
The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Actin-depolymerizing factor; ADF; ADFBrevin; AGEL; Brevin; DKFZp313L0718; Gelsolin; RP11-477J21.1
Gene Aliases: ADF; AGEL; Gsb; GSN
UniProt ID: (Human) P06396, (Rat) Q68FP1, (Mouse) P13020
Entrez Gene ID: (Human) 2934, (Rat) 296654, (Mouse) 227753
phagocytosis, engulfment
aging
positive regulation of gene expression
oligodendrocyte development
striated muscle atrophy
extracellular matrix disassembly
actin filament polymerization
protein destabilization
tissue regeneration
sequestering of actin monomers
cellular protein metabolic process
actin nucleation
response to ethanol
negative regulation of viral entry into host cell
phosphatidylinositol-mediated signaling
actin filament severing
barbed-end actin filament capping
positive regulation of actin nucleation
response to folic acid
actin filament capping
cilium morphogenesis
cellular response to cadmium ion
regulation of podosome assembly
actin filament reorganization
renal protein absorption
hepatocyte apoptotic process
positive regulation of keratinocyte apoptotic process
regulation of wound healing, spreading of epidermal cells
regulation of establishment of T cell polarity
regulation of plasma membrane raft polarization
regulation of receptor clustering
positive regulation of protein processing in phagocytic vesicle
amyloid fibril formation
positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
apoptotic process
actin polymerization or depolymerization
vesicle-mediated transport
regulation of cell adhesion
wound healing
cell projection organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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