Proteintech

Geminin Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Geminin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Sodium Citrate buffer (pH 6).

Geminin Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Geminin Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug Geminin antibody (10802-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Geminin Antibody in Immunoprecipitation (IP) — IP result of anti-Geminin (IP:10802-1-AP, 4ug; Detection:10802-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

Product Details

10802-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Immunocytochemistry (ICC/IF)	-	View 8 publications 8 publications
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Geminin Fusion Protein Ag1076 (1-209 aa encoded by BC005185) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.69 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MNPSMKQKQ EEIKENIKNS SVPRRTLKMI QPSASGSLVG RENELSAGLS KRKHRNDHLT STTSSPGVIV PESSENKNLG GVTQESFDLM IKENPSSQYW KEVAEKRRKA LYEALKENEK LHKEIEQKDN EIARLKKENK ELAEVAEHVQ YMAELIERLN GEPLDNFESL DNQEFDSEEE TVEDSLVEDS EIGTCAEGTV SSSTDAKPCI (1-209 aa encoded by BC005185)

Target Information

GMNN inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ex vivo assays to predict enhanced chemosensitization by hyperthermia in urothelial cancer of the bladder.

PloS one

van den Tempel N,Naipal KAT,Raams A,van Gent DC,Franckena M,Boormans JL,Kanaar R

Mon May 13 00:00:00 EDT 2019

A RAD51 assay feasible in routine tumor samples calls PARP inhibitor response beyond BRCA mutation.

EMBO molecular medicine

Castroviejo-Bermejo M,Cruz C,Llop-Guevara A,Gutiérrez-Enríquez S,Ducy M,Ibrahim YH,Gris-Oliver A,Pellegrino B,Bruna A,Guzmán M,Rodríguez O,Grueso J,Bonache S,Moles-Fernández A,Villacampa G,Viaplana C,Gómez P,Vidal M,Peg V,Serres-Créixams X,Dellaire G,Simard J,Nuciforo P,Rubio IT,Dienstmann R,Barrett JC,Caldas C,Baselga J,Saura C,Cortés J,Déas O,Jonkers J,Masson JY,Cairo S,Judde JG,O'Connor MJ,Díez O,Balmaña J,Serra V

Sat Dec 01 00:00:00 EST 2018

BRCA1 Mutation-Specific Responses to 53BP1 Loss-Induced Homologous Recombination and PARP Inhibitor Resistance.

Cell reports

Nacson J,Krais JJ,Bernhardy AJ,Clausen E,Feng W,Wang Y,Nicolas E,Cai KQ,Tricarico R,Hua X,DiMarcantonio D,Martinez E,Zong D,Handorf EA,Bellacosa A,Testa JR,Nussenzweig A,Gupta GP,Sykes SM,Johnson N

Tue Sep 25 00:00:00 EDT 2018

Combined B, T and NK Cell Deficiency Accelerates Atherosclerosis in BALB/c Mice.

PloS one

Cheng F,Twardowski L,Reifenberg K,Winter K,Canisius A,Pross E,Fan J,Schmitt E,Shultz LD,Lackner KJ,Torzewski M

Tue Aug 08 00:00:00 EDT 2017

Cumulative defects in DNA repair pathways drive the PARP inhibitor response in high-grade serous epithelial ovarian cancer cell lines.

Oncotarget

Fleury H,Carmona E,Morin VG,Meunier L,Masson JY,Tonin PN,Provencher D,Mes-Masson AM

Tue Jun 20 00:00:00 EDT 2017

APC/C^CDC20 and APC/C play pivotal roles in the process of embryonic development in Artemia sinica.

Scientific reports

Zhang M,Yao F,Luan H,Zhao W,Jing T,Zhang S,Hou L,Zou X

Mon Dec 19 00:00:00 EST 2016

In Vivo Stabilization of a Gastrin-Releasing Peptide Receptor Antagonist Enhances PET Imaging and Radionuclide Therapy of Prostate Cancer in Preclinical Studies.

Theranostics

Chatalic KL,Konijnenberg M,Nonnekens J,de Blois E,Hoeben S,de Ridder C,Brunel L,Fehrentz JA,Martinez J,van Gent DC,Nock BA,Maina T,van Weerden WM,de Jong M

Thu Oct 06 00:00:00 EDT 2016

Delayed DNA double-strand break repair following platin-based chemotherapy predicts treatment response in head and neck squamous cell carcinoma.

British journal of cancer

Bhide SA,Thway K,Lee J,Wong K,Clarke P,Newbold KL,Nutting CM,Harrington KJ

Tue Sep 27 00:00:00 EDT 2016

Hyperthermia Synergizes with Chemotherapy by Inhibiting PARP1-Dependent DNA Replication Arrest.

Cancer research

Schaaf L,Schwab M,Ulmer C,Heine S,Mürdter TE,Schmid JO,Sauer G,Aulitzky WE,van der Kuip H

Sun May 15 00:00:00 EDT 2016

The BRCA1-Δ11q Alternative Splice Isoform Bypasses Germline Mutations and Promotes Therapeutic Resistance to PARP Inhibition and Cisplatin.

Cancer research

Wang Y,Bernhardy AJ,Cruz C,Krais JJ,Nacson J,Nicolas E,Peri S,van der Gulden H,van der Heijden I,O'Brien SW,Zhang Y,Harrell MI,Johnson SF,Candido Dos Reis FJ,Pharoah PD,Karlan B,Gourley C,Lambrechts D,Chenevix-Trench G,Olsson H,Benitez JJ,Greene MH,Gore M,Nussbaum R,Sadetzki S,Gayther SA,Kjaer SK,D'Andrea AD,Shapiro GI,Wiest DL,Connolly DC,Daly MB,Swisher EM,Bouwman P,Jonkers J,Balmaña J,Serra V,Johnson N

Sun May 01 00:00:00 EDT 2016

Cell cycle and apoptosis regulatory proteins, proliferative markers, cell signaling molecules, CD209, and decorin immunoreactivity in low-grade myxofibrosarcoma and myxoma.

Virchows Archiv : an international journal of pathology

Cates JM,Memoli VA,Gonzalez RS

Sat Aug 01 00:00:00 EDT 2015

Whole genomes redefine the mutational landscape of pancreatic cancer.

Nature

Waddell N,Pajic M,Patch AM,Chang DK,Kassahn KS,Bailey P,Johns AL,Miller D,Nones K,Quek K,Quinn MC,Robertson AJ,Fadlullah MZ,Bruxner TJ,Christ AN,Harliwong I,Idrisoglu S,Manning S,Nourse C,Nourbakhsh E,Wani S,Wilson PJ,Markham E,Cloonan N,Anderson MJ,Fink JL,Holmes O,Kazakoff SH,Leonard C,Newell F,Poudel B,Song S,Taylor D,Waddell N,Wood S,Xu Q,Wu J,Pinese M,Cowley MJ,Lee HC,Jones MD,Nagrial AM,Humphris J,Chantrill LA,Chin V,Steinmann AM,Mawson A,Humphrey ES,Colvin EK,Chou A,Scarlett CJ,Pinho AV,Giry-Laterriere M,Rooman I,Samra JS,Kench JG,Pettitt JA,Merrett ND,Toon C,Epari K,Nguyen NQ,Barbour A,Zeps N,Jamieson NB,Graham JS,Niclou SP,Bjerkvig R,Grützmann R,Aust D,Hruban RH,Maitra A,Iacobuzio-Donahue CA,Wolfgang CL,Morgan RA,Lawlor RT,Corbo V,Bassi C,Falconi M,Zamboni G,Tortora G,Tempero MA,Gill AJ,Eshleman JR,Pilarsky C,Scarpa A,Musgrove EA,Pearson JV,Biankin AV,Grimmond SM

Thu Feb 26 00:00:00 EST 2015

Cell cycle kinetic analysis of colorectal neoplasms using a new automated immunohistochemistry-based cell cycle detection method.

Medicine

Tomono A,Itoh T,Yanagita E,Imagawa N,Kakeji Y

Thu Jan 01 00:00:00 EST 2015

Functional ex vivo assay to select homologous recombination-deficient breast tumors for PARP inhibitor treatment.

Clinical cancer research : an official journal of the American Association for Cancer Research

Naipal KA,Verkaik NS,Ameziane N,van Deurzen CH,Ter Brugge P,Meijers M,Sieuwerts AM,Martens JW,O'Connor MJ,Vrieling H,Hoeijmakers JH,Jonkers J,Kanaar R,de Winter JP,Vreeswijk MP,Jager A,van Gent DC

Mon Sep 15 00:00:00 EDT 2014

A marker of homologous recombination predicts pathologic complete response to neoadjuvant chemotherapy in primary breast cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Graeser M,McCarthy A,Lord CJ,Savage K,Hills M,Salter J,Orr N,Parton M,Smith IE,Reis-Filho JS,Dowsett M,Ashworth A,Turner NC

Wed Dec 15 00:00:00 EST 2010

Bioinformatics

Protein Aliases: Geminin; Geminin GMNN; OTTHUMP00000039393; RP3 369A17.3

Gene Aliases: AW546347; Gem; GMNN; MGORS6

UniProt ID: (Human) O75496, (Mouse) O88513

Entrez Gene ID: (Human) 51053, (Rat) 291137, (Mouse) 57441

Function(s)

protein complex assembly negative regulation of DNA replication organ morphogenesis negative regulation of cell cycle negative regulation of transcription, DNA-templated cell cycle regulation of DNA replication

Process(es)

transcription corepressor activity protein binding histone deacetylase binding repressing transcription factor binding transcription regulatory region DNA binding

Geminin Polyclonal Antibody

Product Details

10802-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Immunocytochemistry (ICC/IF)

Immunohistochemistry (IHC)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!