Invitrogen

Ghrelin Polyclonal Antibody

Product Image Gallery

Ghrelin Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of GHRL was performed using 70% confluent log phase MKN-45 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ghrelin Rabbit Polyclonal Antibody (Product # PA1-1070) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Ghrelin Antibody in Western Blot (WB) — Western blot detection of Obestatin using Product # PA1-1070.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

2 µg/mL

Immunofluorescence (IF)

2 µg/mL

Western Blot (WB)

1:1000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues F(1) N A P F D V G I K L S G A Q Y Q Q H G R A L(23) of the rat Obestatin gene.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_1074594

Product Specific Information

PA1-1070 detects Obestatin in mouse and rat samples. This product does not detect endogenous levels of the hormone in rat stomach.

PA1-1070 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~2.5 kDa protein representing synthetic Obestatin.

The PA1-1070 immunogen is a synthetic peptide corresponding to residues F(1) N A P F D V G I K L S G A Q Y Q Q H G R A L(23) of the rat Obestatin gene. This sequence is conserved in mouse and only one amino acid residue difference in human. This peptide (Cat. # PEP-290) is available for use in neutralization and control experiments.

Target Information

GHRL encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an the ligand for the growth hormone secretagogue receptor type 1 and is involved in regulating growth hormone release. Ghrelin has an appeptite-stimulating effect and induces adiposity and stimulates gastric acid secretion. Obestatin may be the ligand for the orphan G protein-coupled receptor GPR39 and is involved in satiety and can have an appetite-reducing effect resulting in decreased food intake.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 2210006E23Rik; Appetite-regulating hormone; Ghrelin; ghrelin, growth hormone secretagogue receptor ligand; Ghrelin-27; Ghrelin-28; ghrelin/obestatin preprohormone; Growth hormone secretagogue; Growth hormone-releasing peptide; In2c-preproghrelin; m46; Motilin-related peptide; MTLRP; MTLRPAP; Obestatin; Obestatin-13; Obestatin-23; prepro-appetite regulatory hormone; preproghrelin; proghrelin; Protein M46

Gene Aliases: 2210006E23Rik; Ghr; GHRL; m46; MTLRP; MTLRPAP; UNQ524/PRO1066

UniProt ID: (Human) Q9UBU3, (Rat) Q9QYH7, (Mouse) Q9EQX0

Entrez Gene ID: (Human) 51738, (Rat) 59301, (Mouse) 58991

Function(s)