|Tested species reactivity||Human|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from C-terminus of human GluR1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chicken, mouse and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is brain tissue.
Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: lonotropic receptors, and metabotropic recetors. Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR-17, NMDA1-3, KAI-2), which comprise ligand-gated calcium channels. Metabotroic glutamate receptors are G-protein coupled receptors, which when activated can activate phopholipases and adenylylcyclase, depending on the neuron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nucleus Accumbens AMPA Receptors Are Necessary for Morphine-Withdrawal-Induced Negative-Affective States in Rats.
PA1-37776 was used in western blot to learn the necessity for nucleus accumbens AMPA receptors morphine-withdrawal-induced negative-affective states in rats
|Russell SE,Puttick DJ,Sawyer AM,Potter DN,Mague S,Carlezon WA,Chartoff EH||The Journal of neuroscience : the official journal of the Society for Neuroscience (36:5748)||2016|
AMPA receptor upregulation in the nucleus accumbens shell of cocaine-sensitized rats depends upon S-nitrosylation of stargazin.
PA1-37776 was used in western blot to study the role of stargazin N-nitrosylation in the upregulation of the AMPA receptor in the nucleus accumbens shell of cocaine-sensitized rats
|Selvakumar B,Campbell PW,Milovanovic M,Park DJ,West AR,Snyder SH,Wolf ME||Neuropharmacology (77:28)||2014|
Kalirin-7 mediates cocaine-induced AMPA receptor and spine plasticity, enabling incentive sensitization.
PA1-37776 was used in western blot to study the role of kalirin-7 in the mechanism by which cocaine-induced AMPAR and spine upregulation lead to incentive sensitization
|Wang X,Cahill ME,Werner CT,Christoffel DJ,Golden SA,Xie Z,Loweth JA,Marinelli M,Russo SJ,Penzes P,Wolf ME||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:11012)||2013|
|Not Applicable||0.5 µg/ml||
Local potentiation of excitatory synapses by serotonin and its alteration in rodent models of depression.
PA1-37776 was used in western blot to elucidate how serotonin alleviates depression
|Cai X,Kallarackal AJ,Kvarta MD,Goluskin S,Gaylor K,Bailey AM,Lee HK,Huganir RL,Thompson SM||Nature neuroscience (16:464)||2013|
Different roles of BDNF in nucleus accumbens core versus shell during the incubation of cue-induced cocaine craving and its long-term maintenance.
PA1-37776 was used in western blot to study the role of BDNF in the incubation of cocaine craving following withrawal from extended self-administration
|Li X,DeJoseph MR,Urban JH,Bahi A,Dreyer JL,Meredith GE,Ford KA,Ferrario CR,Loweth JA,Wolf ME||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:1130)||2013|
A protein cross-linking assay for measuring cell surface expression of glutamate receptor subunits in the rodent brain after in vivo treatments.
PA1-37776 was used in western blot to develop an assay for measuring cell surface-expressed glutamate receptors utilizing cell-impermeant protein cross-linking agents
|Boudreau AC,Milovanovic M,Conrad KL,Nelson C,Ferrario CR,Wolf ME||Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.] (Chapter 5:-)||2012|
Brain-derived neurotrophic factor rapidly increases AMPA receptor surface expression in rat nucleus accumbens.
PA1-37776 was used in western blot to investigate the effect of BDNF on AMPAR expression in rat nucleus accumbens
|Li X,Wolf ME||The European journal of neuroscience (34:190)||2011|
Calcium-permeable AMPA receptors are present in nucleus accumbens synapses after prolonged withdrawal from cocaine self-administration but not experimenter-administered cocaine.
PA1-37776 was used in western blot to investigate the role of calcium-permeable AMPA receptors during prolonged withdrawal from cocaine self-administration
|McCutcheon JE,Wang X,Tseng KY,Wolf ME,Marinelli M||The Journal of neuroscience : the official journal of the Society for Neuroscience (31:5737)||2011|
Distribution of AMPA receptor subunits and TARPs in synaptic and extrasynaptic membranes of the adult rat nucleus accumbens.
PA1-37776 was used in western blot to study the expression of AMPA receptors and transmembrane AMPA receptor regulatory proteins in adult rat nucleus accumbens
|Ferrario CR,Loweth JA,Milovanovic M,Wang X,Wolf ME||Neuroscience letters (490:180)||2011|
Effects of acute cocaine or dopamine receptor agonists on AMPA receptor distribution in the rat nucleus accumbens.
PA1-37776 was used in western blot to investigate the influence of acute cocaine on AMPAR surface expression in the rat nucleus accumbens
|Ferrario CR,Li X,Wolf ME||Synapse (New York, N.Y.) (65:54)||2011|
Alterations in AMPA receptor subunits and TARPs in the rat nucleus accumbens related to the formation of Ca²¿-permeable AMPA receptors during the incubation of cocaine craving.
PA1-37776 was used in immunohistochemistry to investigate the mechanism for the incubation of cocaine craving
|Ferrario CR,Loweth JA,Milovanovic M,Ford KA,Galiñanes GL,Heng LJ,Tseng KY,Wolf ME||Neuropharmacology (61:1141)||2011|