Invitrogen

GluR1 Polyclonal Antibody

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GluR1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of GluR1 using anti-GluR1 Polyclonal Antibody (Product # PA5-32425) in Brain Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:50.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Western Blot (WB)

Assay dependent

Published Applications

Western Blot (WB)

See 11 publications below

Immunohistochemistry (IHC)

See 1 publication below

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide from C-terminus of human GluR1.

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2549895

Product Specific Information

This antibody is predicted to react with chicken, mouse and rat based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is brain tissue.

Target Information

GluR1 (glutamate receptor 1) is an ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter, L-glutamate, induces a conformation change that leads to the opening of the cation channel and converts the chemical signal to an electrical impulse. Then, the receptor desensitizes rapidly and enters a transiet inactive state characterized by the presence of a bound agonist. In the presence of CACNG4, CACNG7, or CACNG8 GluR1 shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AMPA 1; AMPA-selective glutamate receptor 1; GluA1; GluR-1; GluR-A; GluR-K1; Glutamate receptor 1; Glutamate receptor ionotropic, AMPA 1; glutamate receptor, ionotropic, AMPA 1; GPRC1A; GRM1A; metabotropic glutamate receptor 1; mGlu1; MGLUR1; MGLUR1A

Gene Aliases: GluA1; GLUH1; GLUR1; GLURA; GRIA1; HBGR1

UniProt ID: (Human) P42261

Entrez Gene ID: (Human) 2890

Molecular Function: ion channel ionotropic glutamate receptor ligand-gated ion channel receptor transporter