Invitrogen

GluR2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

8 Published Figures

5 References

View all (26) GluR2 antibodies

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Protocols

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GluR2 Antibody in Western Blot (WB) — Western blot was performed using GluR2 Polyclonal Antibody (Product # PA1-4659) and a 100kDa band corresponding to GluR2 was observed across Mouse and Rat tissues tested except Mouse Liver along with an uncharacterized band at ~30kDa. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Cerebellum (Lane 2), Rat Brain (Lane 3) and Mouse Liver (Lane 4) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

GluR2 Antibody — Figure 5 Effects of a history of food restriction (FR), with and without episodic intake of 10% sucrose, on abundance of pSer845-GluA1, GluA1, GluA2, and GluA3 in nucleus accumbens postsynaptic density. Following densitometry, intensities of bands corresponding to the target AMPA receptor protein for each sample were divided by the intensities of the corresponding alpha-tubulin bands. Results (mean +- s.e.m.) are expressed in comparison to the normalized control, defined as the AL group with a history of episodic access to tap water and a terminal treatment of 15-min access to tap water. Representative immunoblots are included in sequence corresponding to bars immediately above. n=15 per group, with 3 NAc pooled per tube for fractionation. * greater than all AL groups and the previously FR group with access to tap water, p at least <.05; + greater than all AL groups, p at least <.05. {TM}

Product Details

PA1-4659

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 5 publications 5 publications

Product Specifications
Species Reactivity	Rat
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to amino acid residues specific to rat GluR2 conjugated to KLH
Conjugate	Unconjugated
Form	Liquid
Purification	Affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2113757

Product Specific Information

This antibody is predicted to react with chicken, human, mouse, non-human primate and zebrafish based on 100% sequence homology.

This antibody is specific for the ~100 kDa GluR2 protein.

Target Information

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5 -methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct Glutamate Receptor Subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR2 subunit is thought to play a key role in forms of synaptic plasticity such as LTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Food restriction induces synaptic incorporation of calcium-permeable AMPA receptors in nucleus accumbens.

The European journal of neuroscience

Ouyang J,Carcea I,Schiavo JK,Jones KT,Rabinowitsch A,Kolaric R,Cabeza de Vaca S,Froemke RC,Carr KD

PA14659 was used in western blot to assess whether food restriction alters AMPA receptor subunit abundance in the nucleus accumbens postsynaptic density

Wed Mar 01 00:00:00 EST 2017

Nucleus accumbens AMPA receptor involvement in cocaine-conditioned place preference under different dietary conditions in rats.

Psychopharmacology

Zheng D,Cabeza de Vaca S,Jurkowski Z,Carr KD

PA1-4659 was used in western blot to examine the effect of food restriction on cocaine place preference conditioning in rats

Wed Jul 01 00:00:00 EDT 2015

Episodic sucrose intake during food restriction increases synaptic abundance of AMPA receptors in nucleus accumbens and augments intake of sucrose following restoration of ad libitum feeding.

Neuroscience

Peng XX,Lister A,Rabinowitsch A,Kolaric R,Cabeza de Vaca S,Ziff EB,Carr KD

PA1-4659 was used in western blot to test the hypothesis that episodic "breakthrough" intake of forbidden food during dieting interacts with upregulated mechanisms of synaptic plasticity to increase reward-driven feeding

Thu Jun 04 00:00:00 EDT 2015

Involvement of nucleus accumbens AMPA receptor trafficking in augmentation of D- amphetamine reward in food-restricted rats.

Psychopharmacology

Peng XX,Cabeza de Vaca S,Ziff EB,Carr KD

PA1-4659 was used in western blot to study the effects of food restriction on AMPAR trafficking in the nucleus accumbens in response to D-amphetamine

Fri Aug 01 00:00:00 EDT 2014

Effects of food restriction and sucrose intake on synaptic delivery of AMPA receptors in nucleus accumbens.

Synapse (New York, N.Y.)

Peng XX,Ziff EB,Carr KD

PA1-4659 was used in western blot to study the relationship between dietary intake and synaptic delivery of AMPA receptors in nucleus accumbens

Sat Oct 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: AMPA-selective glutamate receptor 2; GluA2; GluR-2; GluR-B; GluR-K2; glutamate receptor; Glutamate receptor 2; glutamate receptor B; Glutamate receptor ionotropic, AMPA 2; OTTHUMP00000165324; OTTHUMP00000220942; OTTHUMP00000220943

Gene Aliases: GluA2; gluR-B; GluR-K2; GluR2; Gria2

UniProt ID: (Rat) P19491

Entrez Gene ID: (Rat) 29627

Molecular Function: transmembrane signal receptor

Function(s)

regulation of receptor recycling chemical synaptic transmission response to lithium ion receptor internalization ion transmembrane transport ionotropic glutamate receptor signaling pathway establishment of protein localization positive regulation of synaptic transmission protein tetramerization regulation of synaptic transmission, glutamatergic response to fungicide

Process(es)

receptor activity ionotropic glutamate receptor activity AMPA glutamate receptor activity extracellular-glutamate-gated ion channel activity calcium channel regulator activity protein binding kainate selective glutamate receptor activity protein kinase binding PDZ domain binding identical protein binding

GluR2 Polyclonal Antibody

Product Details

PA1-4659

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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