|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acid residues specific to rat GluR2 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
This antibody is predicted to react with chicken, human, mouse, non-human primate and zebrafish based on 100% sequence homology.
This antibody is specific for the ~100 kDa GluR2 protein.
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5 -methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct Glutamate Receptor Subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR2 subunit is thought to play a key role in forms of synaptic plasticity such as LTD (Chung et al., 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nucleus accumbens AMPA receptor involvement in cocaine-conditioned place preference under different dietary conditions in rats.
PA1-4659 was used in western blot to examine the effect of food restriction on cocaine place preference conditioning in rats
|Zheng D,Cabeza de Vaca S,Jurkowski Z,Carr KD||Psychopharmacology (232:2313)||2015|
Episodic sucrose intake during food restriction increases synaptic abundance of AMPA receptors in nucleus accumbens and augments intake of sucrose following restoration of ad libitum feeding.
PA1-4659 was used in western blot to test the hypothesis that episodic "breakthrough" intake of forbidden food during dieting interacts with upregulated mechanisms of synaptic plasticity to increase reward-driven feeding
|Peng XX,Lister A,Rabinowitsch A,Kolaric R,Cabeza de Vaca S,Ziff EB,Carr KD||Neuroscience (295:58)||2015|
Involvement of nucleus accumbens AMPA receptor trafficking in augmentation of D- amphetamine reward in food-restricted rats.
PA1-4659 was used in western blot to study the effects of food restriction on AMPAR trafficking in the nucleus accumbens in response to D-amphetamine
|Peng XX,Cabeza de Vaca S,Ziff EB,Carr KD||Psychopharmacology (231:3055)||2014|
Effects of food restriction and sucrose intake on synaptic delivery of AMPA receptors in nucleus accumbens.
PA1-4659 was used in western blot to study the relationship between dietary intake and synaptic delivery of AMPA receptors in nucleus accumbens
|Peng XX,Ziff EB,Carr KD||Synapse (New York, N.Y.) (65:1024)||2011|