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GlyT2 Antibody (PA5-116532) in WB
Western blot analysis of GlyT2 in extracts of various cell lines. Samples were incubated with GlyT2 Polyclonal antibody (Product # PA5-116532) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-116532
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SLC6A5 (NP_0042023)
Conjugate
Unconjugated
Form
Liquid
Concentration
2.20 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901163
Product Specific Information
Positive Samples: SH-SY5Y, U-937, U-87MG, U-251MG, Mouse brain, Mouse liver, Rat brain
Immunogen sequence: MDCSAPKEMN KLPANSPEAA AAQGHPDGPC APRTSPEQEL PAAAAPPPPR VPRSASTGAQ TFQSADARAC EAERPGVGSC KLSSPRAQAA SAALRDLREA QGAQASPPPG SSGPGNALHC KIPFLRGPEG DANVSVGKGT LERNNTPVVG WVNMSQSTVV LGTDGITSVL PGSVATVATQ EDEQGDENKA RGNWSSKLDF
Target Information
This gene encodes a sodium- and chloride-dependent glycine neurotransmitter transporter. This integral membrane glycoprotein is responsible for the clearance of extracellular glycine during glycine-mediated neurotransmission.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glycine transporter 2; GlyT-2; slc6a5; Sodium- and chloride-dependent glycine transporter 2; solute carrier family 6 (neurotransmitter transporter, glycine), member 5; Solute carrier family 6 member 5
Gene Aliases: GLYT-2; GLYT2; HKPX3; NET1; prestin; SLC6A5
UniProt ID: (Human) Q9Y345, (Rat) P58295
Entrez Gene ID: (Human) 9152, (Rat) 171148, (Mouse) 104245
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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