Proteintech

Glypican 1 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Glypican 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 16700-1-AP (Glypican 1 antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Glypican 1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 16700-1-AP (Glypican 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Glypican 1 Antibody in Immunoprecipitation (IP) — IP result of anti-Glypican 1 (IP:16700-1-AP, 4ug; Detection:16700-1-AP 1:600) with BxPC-3 cells lysate 3100 ug.

Product Details

16700-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Glypican 1 Fusion Protein Ag10095 (208-558 aa encoded by BC051279)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: ELRLRATRA FVAARSFVQG LGVASDVVRK VAQVPLGPEC SRAVMKLVYC AHCLGVPGAR PCPDYCRNVL KGCLANQADL DAEWRNLLDS MVLITDKFWG TSGVESVIGS VHTWLAEAIN ALQDNRDTLT AKVIQGCGNP KVNPQGPGPE EKRRRGKLAP RERPPSGTLE KLVSEAKAQL RDVQDFWISL PGTLCSEKMA LSTASDDRCW NGMARGRYLP EVMGDGLANQ INNPEVEVDI TKPDMTIRQQ IMQLKIMTNR LRSAYNGNDV DFQDASDDGS GSGSGDGCLD DLCGRKVSRK SSSSRTPLTH ALPGLSEQEG QKTSAASCPQ PPTFLLPLLL FLALTVARPR WR (208-558 aa encoded by BC051279)

Target Information

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GPC1 Is Associated with Poor Prognosis and Treg Infiltration in Colon Adenocarcinoma.

Computational and mathematical methods in medicine

Liu Y,Ren H,Yang MQ,Li JY

Wed Sep 28 00:00:00 EDT 2022

GPC1 promotes the growth and migration of colorectal cancer cells through regulating the TGF-β1/SMAD2 signaling pathway.

PloS one

Lu F,Chen S,Shi W,Su X,Wu H,Liu M

Thu Jun 09 00:00:00 EDT 2022

GPC1 promotes the growth and migration of colorectal cancer cells through regulating the TGF-β1/SMAD2 signaling pathway.

PloS one

Lu F,Chen S,Shi W,Su X,Wu H,Liu M

Thu Jun 09 00:00:00 EDT 2022

The Glypican-1/HGF/C-Met and Glypican-1/VEGF/VEGFR2 Ternary Complexes Regulate Hair Follicle Angiogenesis.

Frontiers in cell and developmental biology

Colin-Pierre C,Berthélémy N,Belloy N,Danoux L,Bardey V,Rivet R,Mine S,Jeanmaire C,Maquart FX,Ramont L,Brézillon S

Tue Dec 28 00:00:00 EST 2021

The Glypican-1/HGF/C-Met and Glypican-1/VEGF/VEGFR2 Ternary Complexes Regulate Hair Follicle Angiogenesis.

Frontiers in cell and developmental biology

Colin-Pierre C,Berthélémy N,Belloy N,Danoux L,Bardey V,Rivet R,Mine S,Jeanmaire C,Maquart FX,Ramont L,Brézillon S

Tue Dec 28 00:00:00 EST 2021

The Glypican-1/HGF/C-Met and Glypican-1/VEGF/VEGFR2 Ternary Complexes Regulate Hair Follicle Angiogenesis.

Frontiers in cell and developmental biology

Colin-Pierre C,Berthélémy N,Belloy N,Danoux L,Bardey V,Rivet R,Mine S,Jeanmaire C,Maquart FX,Ramont L,Brézillon S

Tue Dec 28 00:00:00 EST 2021

Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α.

Nature communications

Le Thomas A,Ferri E,Marsters S,Harnoss JM,Lawrence DA,Zuazo-Gaztelu I,Modrusan Z,Chan S,Solon M,Chalouni C,Li W,Koeppen H,Rudolph J,Wang W,Wu TD,Walter P,Ashkenazi A

Wed Dec 15 00:00:00 EST 2021

Heparan sulfate proteoglycan glypican-1 and PECAM-1 cooperate in shear-induced endothelial nitric oxide production.

Scientific reports

Bartosch AMW,Mathews R,Mahmoud MM,Cancel LM,Haq ZS,Tarbell JM

Mon May 31 00:00:00 EDT 2021

Overexpression of Annexin A2 promotes proliferation by forming a Glypican 1/c-Myc positive feedback loop: prognostic significance in human glioma.

Cell death & disease

Li X,Nie S,Lv Z,Ma L,Song Y,Hu Z,Hu X,Liu Z,Zhou G,Dai Z,Song T,Liu J,Wang S

Fri Mar 12 00:00:00 EST 2021

Overexpression of Annexin A2 promotes proliferation by forming a Glypican 1/c-Myc positive feedback loop: prognostic significance in human glioma.

Cell death & disease

Li X,Nie S,Lv Z,Ma L,Song Y,Hu Z,Hu X,Liu Z,Zhou G,Dai Z,Song T,Liu J,Wang S

Fri Mar 12 00:00:00 EST 2021

Glypican-1 is a novel immunohistochemical marker to differentiate poorly differentiated squamous cell carcinoma from solid predominant adenocarcinoma of the lung.

Translational lung cancer research

Kai Y,Amatya VJ,Kushitani K,Kambara T,Suzuki R,Fujii Y,Tsutani Y,Miyata Y,Okada M,Takeshima Y

Mon Feb 01 00:00:00 EST 2021

Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle.

Biomolecules

Colin-Pierre C,Untereiner V,Sockalingum GD,Berthélémy N,Danoux L,Bardey V,Mine S,Jeanmaire C,Ramont L,Brézillon S

Sat Jan 30 00:00:00 EST 2021

Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle.

Biomolecules

Colin-Pierre C,Untereiner V,Sockalingum GD,Berthélémy N,Danoux L,Bardey V,Mine S,Jeanmaire C,Ramont L,Brézillon S

Sat Jan 30 00:00:00 EST 2021

IGFBP6 regulates vascular smooth muscle cell proliferation and morphology via cyclin E-CDK2.

Journal of cellular physiology

Wang Z,Qi Y,Wang R,Wu W,Li Z,Wang M,Liu R,Zhang C,Li W,Wang S

Tue Dec 01 00:00:00 EST 2020

SALL4 is a useful marker for pediatric yolk sac tumors.

Pediatric surgery international

Wu P,Luo R,Sun B,Zhao J,Xu Q,Feng S,Chen X,Wang C

Mon Jun 01 00:00:00 EDT 2020

The cancer cell glycocalyx proteoglycan Glypican-1 mediates interstitial flow mechanotransduction to enhance cell migration and metastasis.

Biorheology

Moran H,Cancel LM,Mayer MA,Qazi H,Munn LL,Tarbell JM

Thu Apr 23 00:00:00 EDT 2020

Decreased expression of GPC1 in human skin keratinocytes and epidermis during ageing.

Experimental gerontology

Perrot G,Colin-Pierre C,Ramont L,Proult I,Garbar C,Bardey V,Jeanmaire C,Mine S,Danoux L,Berthélémy N,Maquart FX,Wegrowski Y,Brézillon S

Tue Oct 15 00:00:00 EDT 2019

Decreased expression of GPC1 in human skin keratinocytes and epidermis during ageing.

Experimental gerontology

Perrot G,Colin-Pierre C,Ramont L,Proult I,Garbar C,Bardey V,Jeanmaire C,Mine S,Danoux L,Berthélémy N,Maquart FX,Wegrowski Y,Brézillon S

Tue Oct 15 00:00:00 EDT 2019

miR-324-5p is up regulated in end-stage osteoarthritis and regulates Indian Hedgehog signalling by differing mechanisms in human and mouse.

Matrix biology : journal of the International Society for Matrix Biology

Woods S,Barter MJ,Elliott HR,McGillivray CM,Birch MA,Clark IM,Young DA

Mon Apr 01 00:00:00 EDT 2019

Glypican-1 immunohistochemistry does not separate mesothelioma from pulmonary adenocarcinoma.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Chiu K,Lee L,Cheung S,Churg AM

Sat Sep 01 00:00:00 EDT 2018

Glypican-1 immunohistochemistry does not separate mesothelioma from pulmonary adenocarcinoma.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Chiu K,Lee L,Cheung S,Churg AM

Sat Sep 01 00:00:00 EDT 2018

Glypican-1 immunohistochemistry is a novel marker to differentiate epithelioid mesothelioma from lung adenocarcinoma.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Amatya VJ,Kushitani K,Kai Y,Suzuki R,Miyata Y,Okada M,Takeshima Y

Tue May 01 00:00:00 EDT 2018

High Expression of Glypican-1 Predicts Dissemination and Poor Prognosis in Glioblastomas.

World neurosurgery

Saito T,Sugiyama K,Hama S,Yamasaki F,Takayasu T,Nosaka R,Onishi S,Muragaki Y,Kawamata T,Kurisu K

Fri Sep 01 00:00:00 EDT 2017

High Expression of Glypican-1 Predicts Dissemination and Poor Prognosis in Glioblastomas.

World neurosurgery

Saito T,Sugiyama K,Hama S,Yamasaki F,Takayasu T,Nosaka R,Onishi S,Muragaki Y,Kawamata T,Kurisu K

Fri Sep 01 00:00:00 EDT 2017

Control of organization and function of muscle and tendon by thrombospondin-4.

Matrix biology : journal of the International Society for Matrix Biology

Frolova EG,Drazba J,Krukovets I,Kostenko V,Blech L,Harry C,Vasanji A,Drumm C,Sul P,Jenniskens GJ,Plow EF,Stenina-Adognravi O

Tue Jul 01 00:00:00 EDT 2014

Control of organization and function of muscle and tendon by thrombospondin-4.

Matrix biology : journal of the International Society for Matrix Biology

Frolova EG,Drazba J,Krukovets I,Kostenko V,Blech L,Harry C,Vasanji A,Drumm C,Sul P,Jenniskens GJ,Plow EF,Stenina-Adognravi O

Tue Jul 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: glypican proteoglycan 1; Glypican-1; GPI-anchored cell surface heparan sulfate proteoglycan; HSPG M12; Secreted glypican-1

Gene Aliases: AI462976; glypican; GPC1; HSPG M12; HSPGM12

UniProt ID: (Human) P35052, (Mouse) Q9QZF2, (Rat) P35053

Entrez Gene ID: (Human) 2817, (Mouse) 14733, (Rat) 58920

Function(s)

Schwann cell differentiation heparan sulfate proteoglycan catabolic process myelin assembly negative regulation of fibroblast growth factor receptor signaling pathway retinoid metabolic process glycosaminoglycan biosynthetic process glycosaminoglycan catabolic process axon guidance glycosaminoglycan metabolic process positive regulation of skeletal muscle cell differentiation biological_process

Process(es)

copper ion binding fibroblast growth factor binding laminin binding heparan sulfate proteoglycan binding collagen V binding protein binding

Glypican 1 Polyclonal Antibody

Product Details

16700-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!