Invitrogen

GnRH1 Polyclonal Antibody

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GnRH1 Antibody in Immunohistochemistry (IHC) — Immunlocalization of GnRH in mouse embryo forebrain using Product # PA1-120. The tissue was incubated with PA1-120 for 3 nights.

GnRH1 Antibody in Immunocytochemistry (ICC/IF) — Published figure using GnRH1 polyclonal antibody (Product # PA1-120) in Immunocytochemistry

Product Details

PA1-120

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	1:5,000	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues pyroE(22) H W S Y G L R P G-NH2(32) of mouse GnRH.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_326000

Product Specific Information

PA1-120 detects gonadotropin releasing hormone (GnRH) in mouse brain.

PA1-120 has been successfully used in immunofluorescence and immunohistochemistry procedures. Immunofluorescent staining of GnRH in mouse embryo brain tissue using PA1-120 results in staining of neurons in the rostral forebrain.

In immunohistochemical procedures, 4% paraformaldehyde is recommended as a fixative.

The PA1-120 immunogen is a synthetic peptide corresponding to residues pyroE(22) H W S Y G L R P G-NH2(32) of mouse GnRH. This peptide (Cat. # PEP-168) is available for use in neutralization and control experiments.

Target Information

The Luteinizing Hormone Releasing Hormone (LH-RH) is produced in the hypothalamus and influences the release of Luteinizing Hormone in the pituitary gland. LH-RH is also known as Gonadotropin-releasing hormone (Gn-RH).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Arcuate nucleus neuropeptide coexpression and connections to gonadotrophin-releasing hormone neurones in the female rhesus macaque.

Journal of neuroendocrinology

True C,Takahashi D,Kirigiti M,Lindsley SR,Moctezuma C,Arik A,Smith MS,Kievit P,Grove KL

Published figure using GnRH1 polyclonal antibody (Product # PA1-120) in Immunocytochemistry

Thu Jun 01 00:00:00 EDT 2017

Interleukin-18 and its receptor are expressed in gonadotropin-releasing hormone neurons of mouse and rat forebrain.

Neuroscience letters

Kuwahara-Otani S,Maeda S,Kobayashi K,Minato Y,Tanaka K,Yamanishi K,Hata M,Li W,Hayakawa T,Noguchi K,Okamura H,Yagi H

PA1120 was used in immunohistochemistry - frozen section to determine the distribution of IL-18 signaling components in the hypothalamus of male mice and rats

Mon May 22 00:00:00 EDT 2017

Neurokinin B is critical for normal timing of sexual maturation but dispensable for adult reproductive function in female mice.

Endocrinology

True C,Nasrin Alam S,Cox K,Chan YM,Seminara SB

PA1-120 was used in immunohistochemistry - paraffin section to use Tac2 deficient mice to study neurokinin B-signaling and sexual maturation

Wed Apr 01 00:00:00 EDT 2015

Atrazine inhibits pulsatile gonadotropin-releasing hormone (GnRH) release without altering GnRH messenger RNA or protein levels in the female rat.

Biology of reproduction

Foradori CD,Zimmerman AD,Hinds LR,Zuloaga KL,Breckenridge CB,Handa RJ

PA1-120 was used in immunohistochemistry to study the ability of atrazine to inhibit pulsilate release of gonadotrophin releasing hormone in female rats whilst expression of homone mRNA and protein remain unchanged

Tue Jan 01 00:00:00 EST 2013

Uncovering novel reproductive defects in neurokinin B receptor null mice: closing the gap between mice and men.

Endocrinology

Yang JJ,Caligioni CS,Chan YM,Seminara SB

Published figure using GnRH1 polyclonal antibody (Product # PA1-120) in Immunohistochemistry

Thu Mar 01 00:00:00 EST 2012

Detection and localization of an estrogen receptor beta splice variant protein (ERbeta2) in the adult female rat forebrain and midbrain regions.

The Journal of comparative neurology

Chung WC,Pak TR,Suzuki S,Pouliot WA,Andersen ME,Handa RJ

PA1-120 was used in immunohistochemistry to investigate the expression and distribution of an estrogen receptor beta 2 isoform in adult female rat brain

Tue Nov 20 00:00:00 EST 2007

Bioinformatics

Protein Aliases: GnRH-I; Gonadoliberin I; Gonadoliberin-1; gonadotropin releasing hormone 2; Gonadotropin-releasing hormone I; hypogonadal; LH-RH I; Luliberin I; luteinizing hormone releasing hormone; Luteinizing hormone-releasing hormone I; Lutropin; Progonadoliberin I; Progonadoliberin-1; Prolactin release-inhibiting factor 1; Prolactin release-inhibiting factor I

Gene Aliases: Gnrh; Gnrh1; Gnrh2; hpg; LHRH; Lhrh1; Lnrh

UniProt ID: (Mouse) P13562

Entrez Gene ID: (Mouse) 14714

Molecular Function: peptide hormone intercellular signal molecule

Function(s)

multicellular organism development regulation of gene expression male sex determination negative regulation of immature T cell proliferation negative regulation of apoptotic process response to ethanol response to steroid hormone regulation of ovarian follicle development negative regulation of neuron migration

Process(es)

hormone activity gonadotropin hormone-releasing hormone activity

GnRH1 Polyclonal Antibody

Product Details

PA1-120

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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