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GnRH1 Polyclonal Antibody

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GnRH1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of Hyla cinerea (green treefrog) forebrain using GnRH Polyclonal Antibody (Product # PA1-121). Tissues were blocked in 10% normal goat serum in 0.1M PBS and then stained with PA1-121 at 1:1000 dilution for 48 hours at 4°C followed by a goat anti-rabbit IgG secondary antibody for 60 minutes at room temperature and then an avidin-biotin complex reagent (60min). Staining was developed with DAB substrate for 10 minutes. Data courtesy of Innovators Program.

GnRH1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Published figure using GnRH1 polyclonal antibody (Product # PA1-121) in Immunocytochemistry and Immunohistochemistry

GnRH1 Antibody in Immunocytochemistry (ICC/IF) — Published figure using GnRH1 polyclonal antibody (Product # PA1-121) in Immunocytochemistry

GnRH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Figure 4 GnRH neuron number or axon targeting is unchanged in DIO mice. Coronal sections of the preoptic area ( A , top) and median eminence ( B , bottom) from Ctr and HFD fed male mice, were stained with anti-GnRH antibodies. No diet-mediated changes in GnRH neuron number, nor axon targeting were detected.

GnRH1 Antibody in Immunohistochemistry - Free Floating (IHC (Free)) — Figure 2 GnRH-ir in the bovine POA and ME. (A, G) Low-power images of a representative crystal violet stained sections, red box depicting ME area of photomicrographs. (B-E) POA bipolar and unipolar GnRH-ir cells in DE (B, D) and PE (C, E) animals. (F) Histogram depicting mean number (+-SEM) of GnRH-ir cells identified in the POA of DE and PE animals. (H-I) ME representative images of GnRH-ir fibers in DE (H) and PE (I) animals. (J-K) Histograms depicting mean (+-SEM) arbitrary density units (J) and total area (K) of GnRH-ir fibers in the ME of DE and PE animals.

Product Details

PA1-121

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 16 publications 16 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:1,000	View 3 publications 3 publications
Immunohistochemistry - Free Floating (IHC (Free))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 3 publications 3 publications
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Amphibian, Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues pyroE(22) H W S Y G L R P G-NH2(32) of mouse GnRH.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_325077

Product Specific Information

PA1-121 detects gonadotropin releasing hormone (GnRH) in mouse brain and in amphibian (Hyla cinerea) samples.

PA1-121 has been successfully used in immunohistochemistry procedures. Immunohistochemical staining of GnRH in mouse embryo brain tissue using PA1-121 results in staining of neurons in the organum vasculosum of the lamina terminalis (OVLT).

In immunohistochemical procedures, 4% paraformaldehyde is recommended as a fixative.

The PA1-121 immunogen is a synthetic peptide corresponding to residues pyroE(22) H W S Y G L R P G-NH2(32) of mouse GnRH. This sequence is completely conserved in rat and tree shrew and is 78% conserved in zebrafish. The PA1-121 immunizing peptide (Cat. # PEP-168) is available for use in neutralization and control experiments.

Target Information

The Luteinizing Hormone Releasing Hormone (LH-RH) is produced in the hypothalamus and influences the release of Luteinizing Hormone in the pituitary gland. LH-RH is also known as Gonadotropin-releasing hormone (Gn-RH).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Selective effects of protein 4.1N deficiency on neuroendocrine and reproductive systems.

Scientific reports

Wang H,Parra M,Conboy JG,Hillyer CD,Mohandas N,An X

PA1-121 was used in Immunohistochemistry on paraffin embedded tissues to uncover a novel role for 4.1N in the axis of hypothalamus-pituitary gland-reproductive system.

Mon Oct 12 00:00:00 EDT 2020

Selective effects of protein 4.1N deficiency on neuroendocrine and reproductive systems.

Scientific reports

Wang H,Parra M,Conboy JG,Hillyer CD,Mohandas N,An X

PA1-121 was used in Immunohistochemistry on paraffin embedded tissues to uncover a novel role for 4.1N in the axis of hypothalamus-pituitary gland-reproductive system.

Mon Oct 12 00:00:00 EDT 2020

The Homeodomain Transcription Factors Vax1 and Six6 Are Required for SCN Development and Function.

Molecular neurobiology

Pandolfi EC,Breuer JA,Nguyen Huu VA,Talluri T,Nguyen D,Lee JS,Hu R,Bharti K,Skowronska-Krawczyk D,Gorman MR,Mellon PL,Hoffmann HM

PA1-121 was used in Immunohistochemistry to identify VAX1, and confirm SIX6, as transcription factors required for suprachiasmatic nucleus (SCN) development and function.

Sat Feb 01 00:00:00 EST 2020

Differential CRE Expression in Lhrh-cre and GnRH-cre Alleles and the Impact on Fertility in Otx2-Flox Mice.

Neuroendocrinology

Hoffmann HM,Larder R,Lee JS,Hu RJ,Trang C,Devries BM,Clark DD,Mellon PL

Published figure using GnRH1 polyclonal antibody (Product # PA1-121) in Immunohistochemistry

Thu Dec 26 00:00:00 EST 2019

Diet-Induced Obesity Elicits Macrophage Infiltration and Reduction in Spine Density in the Hypothalami of Male but Not Female Mice.

Frontiers in immunology

Lainez NM,Jonak CR,Nair MG,Ethell IM,Wilson EH,Carson MJ,Coss D

PA1-121 was used in Immunohistochemistry to study the neuro-immune mechanisms underlying sex-specific differences in obesity-induced impairment of the hypothalamic function with potential consequences for reproduction and fertility.

Wed Sep 25 00:00:00 EDT 2019

Sox21 deletion in mice causes postnatal growth deficiency without physiological disruption of hypothalamic-pituitary endocrine axes.

Molecular and cellular endocrinology

Cheung LYM,Okano H,Camper SA

PA1121 was used in immunohistochemistry to explore the role of Sox21 expression in hypothalamic-pituitary development

Thu Jan 05 00:00:00 EST 2017

Distribution and regulation of gonadotropin-releasing hormone, kisspeptin, RF-amide related peptide-3, and dynorphin in the bovine hypothalamus.

PeerJ

Tanco VM,Whitlock BK,Jones MA,Wilborn RR,Brandebourg TD,Foradori CD

PA1-121 was used in immunohistochemistry - free floating to characterize bovine hypothalamus for distribution and regulation of gonadotropin-releasing hormone, RF-amide related peptide-3, kisspeptin, and dynorphin

Fri Mar 25 00:00:00 EDT 2016

Deletion of Vax1 from Gonadotropin-Releasing Hormone (GnRH) Neurons Abolishes GnRH Expression and Leads to Hypogonadism and Infertility.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Hoffmann HM,Trang C,Gong P,Kimura I,Pandolfi EC,Mellon PL

PA1-121 was used in immunohistochemistry - paraffin section to study the abolishment of GnRH expression and leads to hypogonadism and infertility due to a deletion of Vax1 from gonadotropin-releasing hormone (GnRH) neurons

Wed Mar 23 00:00:00 EDT 2016

In Utero Development of Kisspeptin/GnRH Neural Circuitry in Male Mice.

Endocrinology

Kumar D,Periasamy V,Freese M,Voigt A,Boehm U

PA1-121 was used in immunohistochemistry - frozen section to examine the role of kisspeptin/GPR54 in the developing brain

Tue Sep 01 00:00:00 EDT 2015

Olfactomedin 1 Deficiency Leads to Defective Olfaction and Impaired Female Fertility.

Endocrinology

Li R,Diao H,Zhao F,Xiao S,El Zowalaty AE,Dudley EA,Mattson MP,Ye X

PA1-121 was used in immunohistochemistry - frozen section to elucidate the mechanism by which olfactomedin 1 deficiency results in infertility

Tue Sep 01 00:00:00 EDT 2015

Gonadotropin and kisspeptin gene expression, but not GnRH, are impaired in cFOS deficient mice.

Molecular and cellular endocrinology

Xie C,Jonak CR,Kauffman AS,Coss D

Published figure using GnRH1 polyclonal antibody (Product # PA1-121) in Immunohistochemistry

Sat Aug 15 00:00:00 EDT 2015

Chronic oestradiol reduces the dendritic spine density of KNDy (kisspeptin/neurokinin B/dynorphin) neurones in the arcuate nucleus of ovariectomised Tac2-enhanced green fluorescent protein transgenic mice.

Journal of neuroendocrinology

Cholanian M,Krajewski-Hall SJ,McMullen NT,Rance NE

PA1-121 was used in immunohistochemistry to assess the effect of oestrogens on the dendrites of neurons

Wed Apr 01 00:00:00 EDT 2015

Journal of neuroendocrinology

Cholanian M,Krajewski-Hall SJ,McMullen NT,Rance NE

PA1-121 was used in immunohistochemistry to assess the effect of oestrogens on the dendrites of neurons

Wed Apr 01 00:00:00 EDT 2015

Conditional genetic transsynaptic tracing in the embryonic mouse brain.

Journal of visualized experiments : JoVE

Kumar D,Boehm U

PA1-121 was used in immunohistochemistry to describe a method for transsynaptic tracing in mouse embryos using the novel recombinant ROSA26 alleles

Mon Dec 22 00:00:00 EST 2014

Murine arcuate nucleus kisspeptin neurons communicate with GnRH neurons in utero.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kumar D,Freese M,Drexler D,Hermans-Borgmeyer I,Marquardt A,Boehm U

Published figure using GnRH1 polyclonal antibody (Product # PA1-121) in Immunohistochemistry

Wed Mar 05 00:00:00 EST 2014

Murine arcuate nucleus kisspeptin neurons communicate with GnRH neurons in utero.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kumar D,Freese M,Drexler D,Hermans-Borgmeyer I,Marquardt A,Boehm U

Published figure using GnRH1 polyclonal antibody (Product # PA1-121) in Immunohistochemistry

Wed Mar 05 00:00:00 EST 2014

Developmental profile and sexually dimorphic expression of kiss1 and kiss1r in the fetal mouse brain.

Frontiers in endocrinology

Knoll JG,Clay CM,Bouma GJ,Henion TR,Schwarting GA,Millar RP,Tobet SA

Published figure using GnRH1 polyclonal antibody (Product # PA1-121) in Immunofluorescence

Wed Oct 16 00:00:00 EDT 2013

Gene dosage of Otx2 is important for fertility in male mice.

Molecular and cellular endocrinology

Larder R,Kimura I,Meadows J,Clark DD,Mayo S,Mellon PL

Published figure using GnRH1 polyclonal antibody (Product # PA1-121) in Immunohistochemistry

Thu Sep 05 00:00:00 EDT 2013

Lhx2-dependent specification of olfactory sensory neurons is required for successful integration of olfactory, vomeronasal, and GnRH neurons.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Berghard A,Hägglund AC,Bohm S,Carlsson L

PA1-121 was used in immunohistochemistry to study the role of Lhx2 in olfactory sensory neurons in olfactory, vomeronasal and GnRH neuron development

Wed Aug 01 00:00:00 EDT 2012

Genetic identification of GnRH receptor neurons: a new model for studying neural circuits underlying reproductive physiology in the mouse brain.

Endocrinology

Wen S,Götze IN,Mai O,Schauer C,Leinders-Zufall T,Boehm U

PA1-121 was used in immunocytochemistry to evaluate a new mouse model to study the neuronal basis of reproductive physiology in the brain

Fri Apr 01 00:00:00 EDT 2011

Hypothalamic dysregulation and infertility in mice lacking the homeodomain protein Six6.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Larder R,Clark DD,Miller NL,Mellon PL

PA1-121 was used in immunohistochemistry to investigate the role of Six6 in the regulation of GnRH expression

Wed Jan 12 00:00:00 EST 2011

Composition of the migratory mass during development of the olfactory nerve.

The Journal of comparative neurology

Miller AM,Treloar HB,Greer CA

Published figure using GnRH1 polyclonal antibody (Product # PA1-121) in Immunofluorescence

Wed Dec 15 00:00:00 EST 2010

Loss of magel2, a candidate gene for features of Prader-Willi syndrome, impairs reproductive function in mice.

PloS one

Mercer RE,Wevrick R

PA1-121 was used in immunohistochemistry to study the role of Magel2 in reproductive function in mice

Fri May 01 00:00:00 EDT 2009

Necdin, a Prader-Willi syndrome candidate gene, regulates gonadotropin-releasing hormone neurons during development.

Human molecular genetics

Miller NL,Wevrick R,Mellon PL

PA1-121 was used in immunohistochemistry to study how necdin regulates gonadotropin-releasing hormone neurons during development

Thu Jan 15 00:00:00 EST 2009

Heterogeneous expression of the potassium-chloride cotransporter KCC2 in gonadotropin-releasing hormone neurons of the adult mouse.

Endocrinology

Leupen SM,Tobet SA,Crowley WF,Kaila K

PA1-121 was used in immunohistochemistry to investigate the expression of the potassium-chloride cotransporter KCC2 in a subset of adult mouse neurons.

Tue Jul 01 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: GnRH-I; Gonadoliberin I; Gonadoliberin-1; gonadotropin releasing hormone 2; Gonadotropin-releasing hormone I; hypogonadal; LH-RH I; Luliberin I; luteinizing hormone releasing hormone; Luteinizing hormone-releasing hormone I; Lutropin; Progonadoliberin I; Progonadoliberin-1; Prolactin release-inhibiting factor 1; Prolactin release-inhibiting factor I

Gene Aliases: Gnrh; Gnrh1; Gnrh2; hpg; LHRH; Lhrh1; Lnrh

UniProt ID: (Mouse) P13562

Entrez Gene ID: (Mouse) 14714

Molecular Function: peptide hormone intercellular signal molecule

Function(s)

multicellular organism development regulation of gene expression male sex determination negative regulation of immature T cell proliferation negative regulation of apoptotic process response to ethanol response to steroid hormone regulation of ovarian follicle development negative regulation of neuron migration

Process(es)

hormone activity gonadotropin hormone-releasing hormone activity

GnRH1 Polyclonal Antibody

Product Details

PA1-121

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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