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Invitrogen
Goat anti-Rabbit IgG Fab Recombinant Secondary Antibody, Biotin
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FIGURE: 1 / 2
Rabbit IgG Fab Secondary Antibody (SA5-10229) in ELISA
ELISA analysis of Goat anti-Rabbit IgG reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. 500 ng/mL, 200 ng/mL, or 50 ng/mL of Rabbit IgG Fab Recombinant Monoclonal antibody (Product # SA5-10229) was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
Product Details
SA5-10229
Applications
Tested Dilution
Western Blot (WB)
0.1-0.5 µg/mL
ELISA (ELISA)
0.01-0.5 µg/mL
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Goat
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Secondary Antibody
Clone
RMG01
Immunogen
Rabbit IgG.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG Fab
Cross Adsorption
Against Rabbit
Antibody Form
Whole Antibody
Product Specific Information
Specificity: reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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