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Invitrogen
Goat anti-Rabbit IgG Fc Secondary Antibody, TRITC
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FIGURE: 1 / 1
Rabbit IgG Fc Secondary Antibody (A16123) in ICC/IF
Immunofluorescence analysis of Goat anti-Rabbit IgG Fc Secondary Antibody, TRITC conjugate was performed using HepG2 cells stained with alpha-1 antitrypsin Rabbit Polyclonal Primary Antibody (PA516661). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Rabbit primary antibody (1:250 dilution) fo... View More
Product Details
A16123
Applications
Tested Dilution
Immunocytochemistry (ICC/IF)
4 µg/mL
Product Specifications
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobin Fc region
Conjugate
Excitation/Emission Max
552/578 nm
View spectra
Form
Liquid
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Wet ice
RRID
Target
IgG Fc
Antibody Form
Whole Antibody
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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