Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Secondary Antibody, Texas Red-X was performed using Hep G2 cells stained with alpha-1 antitrypsin Rabbit Polyclonal Primary Antibody (PA516661). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Rabbit primary antibody (1:250 dilution) for 3 hours at room temperature. Goat anti-Rabbit IgG (H+L) Secondary Antibody, Texas Red-X (T6391) was used at a concentration of 4µg/ml in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha-1 antitrypsin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG, human serum, mouse IgG, mouse serum and bovine serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 mg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 12 publications below|
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Intracellular Theileria annulata promote invasive cell motility through kinase regulation of the host actin cytoskeleton.
T-6391 was used in immunocytochemistry to elucidate the acquired motility and invasiveness of T. annulata-infected cells.
|Ma M,Baumgartner M||PLoS pathogens (10:null)||2014|
|Not Applicable||Not Cited||A conserved late endosome-targeting signal required for Doa4 deubiquitylating enzyme function.||Amerik A,Sindhi N,Hochstrasser M||The Journal of cell biology (175:825)||2006|
|Not Applicable||Not Cited||Role of bone marrow cells in the healing process of mouse experimental glomerulonephritis.||Hayakawa M,Ishizaki M,Hayakawa J,Migita M,Murakami M,Shimada T,Fukunaga Y||Pediatric research (58:323)||2005|
|Not Applicable||Not Cited||The human antimicrobial peptide LL-37 transfers extracellular DNA plasmid to the nuclear compartment of mammalian cells via lipid rafts and proteoglycan-dependent endocytosis.||Sandgren S,Wittrup A,Cheng F,Jönsson M,Eklund E,Busch S,Belting M||The Journal of biological chemistry (279:17951)||2004|
hGFAP-cre transgenic mice for manipulation of glial and neuronal function in vivo.
T-6391 was used in immunohistochemistry (frozen) to characterize lacZ expression using a transgenic mouse where Cre recombinase under the control of the human glial fibrillary acidic protein promoter.
|Zhuo L,Theis M,Alvarez-Maya I,Brenner M,Willecke K,Messing A||Genesis (New York, N.Y. : 2000) (31:85)||2001|
|Not Applicable||Not Cited||Golgi clusters and vesicles mediate mitotic inheritance independently of the endoplasmic reticulum.||Jokitalo E,Cabrera-Poch N,Warren G,Shima DT||The Journal of cell biology (154:317)||2001|
|Not Applicable||Not Cited||A key role of starburst amacrine cells in originating retinal directional selectivity and optokinetic eye movement.||Yoshida K,Watanabe D,Ishikane H,Tachibana M,Pastan I,Nakanishi S||Neuron (30:771)||2001|
|Not Applicable||Not Cited||The effect of Golgi depletion on exocytic transport.||Pelletier L,Jokitalo E,Warren G||Nature cell biology (2:840)||2000|
|Not Applicable||Not Cited||Requirement for Ras and phosphatidylinositol 3-kinase signaling uncouples the glucocorticoid-induced junctional organization and transepithelial electrical resistance in mammary tumor cells.||Woo PL,Ching D,Guan Y,Firestone GL||The Journal of biological chemistry (274:32818)||1999|
|Not Applicable||Not Cited||Shuttling of CTP:Phosphocholine cytidylyltransferase between the nucleus and endoplasmic reticulum accompanies the wave of phosphatidylcholine synthesis during the G(0) --> G(1) transition.||Northwood IC,Tong AH,Crawford B,Drobnies AE,Cornell RB||The Journal of biological chemistry (274:26240)||1999|
|Not Applicable||Not Cited||Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae.||Rossanese OW,Soderholm J,Bevis BJ,Sears IB,O'Connor J,Williamson EK,Glick BS||The Journal of cell biology (145:69)||1999|
|Not Applicable||Not Cited||LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan.||Banerji S,Ni J,Wang SX,Clasper S,Su J,Tammi R,Jones M,Jackson DG||The Journal of cell biology (144:789)||1999|