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Invitrogen
Goat anti-Rabbit IgG (H+L) Secondary Antibody, AP
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FIGURE: 1 / 1
Rabbit IgG (H+L) Secondary Antibody (31346) in ICC/IF
Immunocytochemistry analysis of Lamin B1 in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 (Product # 28314) in PBS for 30 minutes at room temperature and blocked with Universal Blocker (from Product # 03-6000) for 30 minutes at room temperature. Cells were probed with a Lamin B1 polyclonal antibody (Product # PA5-19468) at a dilution of 1:100 for at least 1 hour at room temperat... View More
Product Details
31346
Applications
Tested Dilution
Western Blot (WB)
1:5,000-1:50,000
Immunohistochemistry (IHC)
1:5,000-1:50,000
Immunocytochemistry (ICC/IF)
1:100-1:1,000
ELISA (ELISA)
1:5,000-1:50,000
Immunoprecipitation (IP)
1:500-1:5,000
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Purified Rabbit IgG, whole molecule
Conjugate
Form
Lyophilized
Concentration
0.6 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 8.0, with 15mg/mL BSA, 50mM sucrose
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Product # 31346 has been successfully used in Western blot, IHC and IP applications.
Product # 31346 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Reconstitute with 0.2 mL of distilled water (1 mg/mL after restoration).
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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