A mouse intestinal section visualized using fluorescent Qdot nanocrystal conjugates. Actin was labeled with a mouse anti-actin monoclonal antibody and visualized using red-fluorescent Qdot655 goat F(ab')2 anti-mouse IgG (Q11022MP, Q11221MP). Laminin was labeled with a rabbit anti-laminin polyclonal antibody and visualized using green-fluorescent Qdot525 goat F(ab')2 anti-rabbit IgG (Q11441MP). Nuclei were stained with blue-fluorescent Hoechst 33342 (H1399, H3570, H21492). Image contributed by Thomas Deerinck and Mark Ellisman, The National Center for Microscopy and Imaging Research, San Diego, CA.<br />
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||F(ab')2 Fragment|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
Qdot nanocrystals are composed of semi-conductor material to generate a fluorescent particle which is exceptionally bright and does not photobleach. Qdot nanocrystals paired with the correct optical filters are as much as 50 times brighter than traditional organic dyes.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Functional characterization and localization of Pneumocystis carinii lanosterol synthase.||Joffrion TM,Collins MS,Sesterhenn T,Cushion MT||Eukaryotic cell (9:107)||2010|
|Not Applicable||Not Cited||Quantitative 3D fluorescence technique for the analysis of en face preparations of arterial walls using quantum dot nanocrystals and two-photon excitation laser scanning microscopy.||Ferrara DE,Weiss D,Carnell PH,Vito RP,Vega D,Gao X,Nie S,Taylor WR||American journal of physiology. Regulatory, integrative and comparative physiology (290:R114)||2006|