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A mouse kidney section visualized with fluorescent Qdot® nanocrystal conjugates. Laminin was labeled with an anti-laminin antibody and visualized using Qdot® 565 goat F(ab')2 anti–rabbit IgG (Q11431MP, Q11432MP). PECAM (platelet/endothelial cell adhesion detection molecule CD31) was detected with a mouse anti–PECAM-1 monoclonal antibody and visualized using red-fluorescent Qdot® 655 goat F(ab')2 anti–mouse IgG (Q11022MP, Q11021MP). Nuclei were stained with blue-fluorescent Hoechst 33342 (H1399, H3570, H21492).
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||F(ab')2 Fragment|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 3 publications below|
Qdot nanocrystals are composed of semi-conductor material to generate a fluorescent particle which is exceptionally bright and does not photobleach. Qdot nanocrystals paired with the correct optical filters are as much as 50 times brighter than traditional organic dyes.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Molecular mapping of tumor heterogeneity on clinical tissue specimens with multiplexed quantum dots.||Liu J,Lau SK,Varma VA,Moffitt RA,Caldwell M,Liu T,Young AN,Petros JA,Osunkoya AO,Krogstad T,Leyland-Jones B,Wang MD,Nie S||ACS nano (4:2755)||2010|
|Not Applicable||Not Cited||The short splice variant of the gamma 2 subunit acts as an external modulator of GABA(A) receptor function.||Boileau AJ,Pearce RA,Czajkowski C||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:4895)||2010|
|Not Applicable||Not Cited||High throughput quantification of protein expression of cancer antigens in tissue microarray using quantum dot nanocrystals.||Ghazani AA,Lee JA,Klostranec J,Xiang Q,Dacosta RS,Wilson BC,Tsao MS,Chan WC||Nano letters (6:2881)||2006|