CAKI cells were plated on coverslips overnight. The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol. Following suppression of background binding using Image-iT® FX Signal enhancer (R37107), cells were incubated with 3 µg/mL anti-ATP synthase subunit IF1 (A21355) for labeling of mitochondria for 30 min at room temperature and washed three times with dPBS, followed by Alexa Fluor® 594 goat anti-rabbit IgG antibody ReadyProbes® reagent (R37117) for 30 min and washed three times in dPBS. Cells were labeled with NucBlue® Live (R37605) and ActinGreen™ 488 ReadyProbes® reagent (R37110) according to protocol. Coverslips were then mounted using ProLong® Gold antifade reagent (P36930). Individual images were acquired on the EVOS® FL Auto imaging system with a 10X objective (AMEP4681) and stitched to a panorama using the tile/stitch function.
|Tested species reactivity||Rabbit|
|Host / Isotype||Goat / IgG|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS with 0.1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against bovine IgG, goat IgG, mouse IgG, rat IgG and human IgG|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.