Description: The eBioDH2 monoclonal antibody reacts with human granulysin, a protein involved in the cytolytic pathway. Granulysin is a member of the saposin-like protein (SAPLIP) family. Two proteins 9 and 15kDa are thought to be post translation modified versions of the 15kDa protein with a change in confirmation. Granulysin is found in NK and activated T cells and the expression is confined to the cytolytic granules which also contains perforin and granzyme. Granulysin with perforn are suspected to function synergistically to kill bacteria and induce apoptosis in nucleated cells.
Applications Reported: This eBioDH2 (DH2) antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunocytochemistry.
Applications Tested: This eBioDH2 (DH2) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
GNLY is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells, which are released upon antigen stimulation. GNLY is present in cytotoxic granules of cytotoxic T lymphocytes and natural killer cells, and it has antimicrobial activity against M. tuberculosis and other organisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Granulysin; 519; D2S69E; LAG-2; LAG2; lymphocyte-activation gene 2; Lymphokine LAG-2; NKG5; Protein NKG5; T-cell activation protein 519; T-lymphocyte activation gene 519; TLA519
Gene Aliases: D2S69E; GNLY; LAG-2; LAG2; NKG5; TLA519
UniProt ID: (Human) P22749
Entrez Gene ID: (Human) 10578