Invitrogen

Granzyme A Polyclonal Antibody

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Granzyme A Antibody in Immunocytochemistry (ICC) — Immunocytochemistry-Immunofluorescence analysis of Granzyme A was performed in U2OS cells using Granzyme A Polyclonal Antibody (Product # PA5-96539) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Granzyme A Antibody in Western Blot (WB) — Western Blot analysis of Granzyme A in extracts of various cell lines using Granzyme A Polyclonal Antibody (Product # PA5-96539) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:50 - 1:200

Immunofluorescence (IF)

1:50 - 1:200

Western Blot (WB)

1:500 - 1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 29-262 of human GZMA.

Conjugate

Unconjugated

Form

Liquid

Concentration

2.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2808341

Product Specific Information

Immunogen sequence: IIGGNEVTPH SRPYMVLLSL DRKTICAGAL IAKDWVLTAA HCNLNKRSQV ILGAHSITRE EPTKQIMLVK KEFPYPCYDP ATREGDLKLL QLTEKAKINK YVTILHLPKK GDDVKPGTMC QVAGWGRTHN SASWSDTLRE VNITIIDRKV CNDRNHYNFN PVIGMNMVCA GSLRGGRDSC NGDSGSPLLC EGVFRGVTSF GLENKCGDPR GPGVYILLSK KHLNWIIMTI KGAV; Positive Samples: Mouse spleen, Mouse thymus, Rat intestine, Rat spleen; Cellular Location: Cytoplasmic granule

Target Information

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Cite this antibody

Bioinformatics

Protein Aliases: Autocrine thymic lymphoma granzyme-like serine protease; BLT esterase; CTL tryptase; CTLA-3; Cytotoxic T-lymphocyte proteinase 1; Cytotoxic T-lymphocyte-associated serine esterase-3; Fragmentin-1; granzyme 1; Granzyme A; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease); granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); Granzyme-1; H factor; Hanukah factor; Hanukah factor serine protease); Hanukkah factor; HF; serine esterase 1; T cell-specific serine protease 1; TSP-1

Gene Aliases: AW494114; Ctla-3; CTLA3; GZMA; Hf; HFSP; Mtsp-1; SE1; TSP-1; TSP1

UniProt ID: (Human) P12544, (Mouse) P11032

Entrez Gene ID: (Human) 3001, (Rat) 266708, (Mouse) 14938

Molecular Function: annexin calcium-binding protein calmodulin enzyme modulator hydrolase intracellular calcium-sensing protein peptide hormone protease protease inhibitor receptor serine protease signaling molecule