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Invitrogen
H-Ras Polyclonal Antibody
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FIGURE: 1 / 6
H-Ras Antibody (PA5-14863) in ICC/IF
Immunofluorescence analysis of HRAS was done on 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were subsequently labeled with HRAS Rabbit Polyclonal Antibody (Product # PA5-14863) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product... View More
Product Details
PA5-14863
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 104-128 amino acids from the central region of human HRAS
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Target Information
Monomeric G protein Ras functions as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to a number of downstream signaling pathways leading to distinct cytoplasmic or nuclear events. Each mammalian cell contains three Ras proto-oncogene coding for closely related Ras proteins: H-, K-, N-Ras. Oncogenic mutations in Ras genes are present in ~30% of all human cancers. Constitutive activation of Ras due to mutations or overexpression stimulates proliferation and inhibition of apoptosis. K-Ras mutations are common in pancreatic, colorectal and nonsmall-cell lung carcinomas; H-Ras mutations are common in bladder, kidney and thyroid carcinomas; N-Ras mutations are found in melanoma, hematological malignancies and hepatocellular carcinaoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-H-ras; c-has/bas p21 protein; GTP- and GDP-binding peptide B; GTPase HRas; GTPase Hras1; H-Ras-1; Ha-Ras; Ha-Ras1 proto-oncoprotein; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; HRAS; p19 H-RasIDX protein; p21ras; Ras family small GTP binding protein H-Ras; small GTP binding protein; transformation gene: oncogene HAMSV; Transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Gene Aliases: C-BAS/HAS; C-H-RAS; C-HA-RAS1; CTLO; H-RASIDX; HAMSV; HRAS; HRAS1; p21ras; RASH1
UniProt ID: (Human) P01112
Entrez Gene ID: (Human) 3265
MAPK cascade
regulation of transcription from RNA polymerase II promoter
endocytosis
chemotaxis
signal transduction
cell surface receptor signaling pathway
small GTPase mediated signal transduction
Ras protein signal transduction
positive regulation of cell proliferation
negative regulation of cell proliferation
insulin receptor signaling pathway
organ morphogenesis
positive regulation of gene expression
negative regulation of gene expression
Schwann cell development
positive regulation of cell migration
positive regulation of interferon-gamma production
regulation of actin cytoskeleton organization
T-helper 1 type immune response
regulation of cell proliferation
myelination
defense response to protozoan
positive regulation of MAPK cascade
negative regulation of neuron apoptotic process
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
fibroblast proliferation
positive regulation of fibroblast proliferation
regulation of long-term neuronal synaptic plasticity
positive regulation of epithelial cell proliferation
T cell receptor signaling pathway
neuron apoptotic process
regulation of cell cycle
adipose tissue development
positive regulation of ERK1 and ERK2 cascade
cellular response to gamma radiation
positive regulation of wound healing
positive regulation of protein targeting to membrane
cellular senescence
oncogene-induced cell senescence
intrinsic apoptotic signaling pathway
regulation of neurotransmitter receptor localization to postsynaptic specialization membrane
positive regulation of ruffle assembly
positive regulation of miRNA metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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