Invitrogen

H-Ras Polyclonal Antibody

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H-Ras Polyclonal Antibody

Product Details

PA5-88424

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human HRAS (NP_0053341)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.03 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2804901

Product Specific Information

Immunogen sequence: MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV REIRQHKLRK LNPPDESGPG CMSCKCVLS; Positive Samples: Mouse brain; Cellular Location: Cell membrane, Cytoplasm, Cytoplasmic side, Golgi apparatus, Golgi apparatus membrane, Lipid-anchor, Lipid-anchor, Nucleus, perinuclear region

Target Information

Monomeric G protein Ras functions as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to a number of downstream signaling pathways leading to distinct cytoplasmic or nuclear events. Each mammalian cell contains three Ras proto-oncogene coding for closely related Ras proteins: H-, K-, N-Ras. Oncogenic mutations in Ras genes are present in ~30% of all human cancers. Constitutive activation of Ras due to mutations or overexpression stimulates proliferation and inhibition of apoptosis. K-Ras mutations are common in pancreatic, colorectal and nonsmall-cell lung carcinomas; H-Ras mutations are common in bladder, kidney and thyroid carcinomas; N-Ras mutations are found in melanoma, hematological malignancies and hepatocellular carcinaoma.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: c-H-ras; c-Ha-ras p21 protein; c-Ha-ras transgene; c-has/bas p21 protein; c-ras-Ki-2 activated oncogene; GTP- and GDP-binding peptide B; GTPase HRas; GTPase Hras1; H-ras 1 protein; H-Ras-1; Ha-Ras; Ha-Ras1 proto-oncoprotein; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; Harvey rat sarcoma virus oncogene 1; p19 H-RasIDX protein; p21ras; Ras family small GTP binding protein H-Ras; transformation gene: oncogene HAMSV; Transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog

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Gene Aliases: C-BAS/HAS; C-H-RAS; c-Ha-ras; C-HA-RAS1; c-rasHa; CTLO; H-ras; H-RASIDX; Ha-ras; HAMSV; Harvey-ras; HRAS; Hras-1; HRAS1; Kras2; p21ras; ras; RASH1

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UniProt ID: (Human) P01112, (Mouse) Q61411

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Entrez Gene ID: (Human) 3265, (Mouse) 15461

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Function(s)
GTPase activity protein binding GTP binding protein C-terminus binding nucleotide binding small GTPase
Process(es)
MAPK cascade positive regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway endocytosis chemotaxis cell cycle arrest mitotic cell cycle checkpoint signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway Ras protein signal transduction axon guidance cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation organ morphogenesis negative regulation of gene expression positive regulation of cell migration positive regulation of interferon-gamma production negative regulation of GTPase activity social behavior Fc-epsilon receptor signaling pathway ERBB2 signaling pathway T-helper 1 type immune response defense response to protozoan positive regulation of MAP kinase activity positive regulation of MAPK cascade negative regulation of neuron apoptotic process positive regulation of GTPase activity positive regulation of DNA replication positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of Ras protein signal transduction ephrin receptor signaling pathway regulation of long-term neuronal synaptic plasticity positive regulation of epithelial cell proliferation T cell receptor signaling pathway leukocyte migration protein heterooligomerization positive regulation of ERK1 and ERK2 cascade positive regulation of wound healing cellular senescence intrinsic apoptotic signaling pathway positive regulation of ruffle assembly positive regulation of actin cytoskeleton reorganization positive regulation of miRNA metabolic process apoptotic process small GTPase mediated signal transduction cell aging positive regulation of gene expression
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