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Invitrogen
H2AFY2 Polyclonal Antibody
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FIGURE: 1 / 10
H2AFY2 Antibody (PA5-115531) in IHC (P)
Immunohistochemistry analysis of H2AFY2 in human kidney cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115531) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.
Product Details
PA5-115531
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human H2AFY2(Accession Q9P0M6), corresponding to amino acid residues E288-F338.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Macro H2A.2.
Target Information
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Core histone macro-H2A.2; core histone macroH2A2.2; H2A histone family member Y2; H2A-variant; Histone macroH2A2; histone mH2A2; mH2A2; unnamed protein product
Gene Aliases: H2AFY2; H2afy3; MACROH2A2; RGD1561371
UniProt ID: (Mouse) Q8CCK0, (Human) Q9P0M6
Entrez Gene ID: (Rat) 361844, (Mouse) 404634, (Human) 55506
molecular_function
chromatin DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
RNA polymerase II regulatory region sequence-specific DNA binding
DNA binding
transcription regulatory region sequence-specific DNA binding
protein binding
structural constituent of chromatin
histone
negative regulation of transcription from RNA polymerase II promoter
regulation of cell growth
nucleosome assembly
brain development
dosage compensation
biological_process
negative regulation of gene expression, epigenetic
establishment of protein localization to chromatin
negative regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter
chromatin modification
positive regulation of keratinocyte differentiation
chromatin organization
dosage compensation by inactivation of X chromosome
heterochromatin assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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