Invitrogen

H4K20me3 Polyclonal Antibody

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H4K20me3 Antibody in Immunocytochemistry (ICC/IF) — Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (tri methyl Lys20) protein stained by Histone H4 (tri methyl Lys20) antibody (Product # PA5-85527) diluted at 1:500. Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by (Product # MA5-31466) diluted at 1:500. Blue: Hoechst 33342 staining.

H4K20me3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — H4K20me3 Polyclonal Antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. H4K20me3 Polyclonal Antibody (Product # PA5-85527) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

H4K20me3 Antibody in Western Blot (WB) — Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract. B. 30 µg HeLa nuclear lysate/extract.15 % SDS-PAGE. Histone H4 (tri methyl Lys20) antibody H4K20me3 Polyclonal Antibody (Product # PA5-85527) dilution: 1:2,000.

H4K20me3 Antibody in Dot blot (DB) — Dot Blot analysis of Tri-Methyl-Histone H4 (Lys20) was performed in various H4K20 methylation peptides using Tri-Methyl-Histone H4 (Lys20) Polyclonal Antibody (Product # PA5-85527) at a dilution of 1:500. Un : H4 protein peptide, Me : H4K20 mono-methylpeptide, 2Me : H4K20 di-methylpeptide, 3Me : H4K20 Tri-methylpeptide.

Product Details

PA5-85527

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Dot blot (DB)	1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4K20me3 (trimethyl Lys20). View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol, 1% BSA
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2792667

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Xenopus laevis (100%).

Positive Control: HeLa, HeLa nuclear.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H4 histone family, member A; H4 histone family, member E; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4 K20; H4K20; H4Lys20me3; HIST1H4; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4j; histone 2, H4; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; Histone Cluster 4; histone family member; Histone H4; Histone H4A; Histone H4K20me3; histone IV, family 2; methyl Histone 3; methyl Histone H3; Tri-methyl H4; Tri-methyl Histone H4; Tri-Methyl-H4 K20; Tri-Methyl-H4 Lys20; Tri-Methyl-Histone H4 K20; Tri-Methyl-Histone H4 Lys20; Trimethyl H4; Trimethyl Histone H4

Gene Aliases: dJ160A22.2; FO108; H4; H4-12; H4-16; H4-53; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4f16; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; Hist1h4m; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; X04652

UniProt ID: (Human) P62805, (Mouse) P62806

Entrez Gene ID: (Human) 8359, (Human) 8366, (Human) 8367, (Human) 8294, (Human) 8363, (Human) 8370, (Human) 121504, (Mouse) 326619, (Mouse) 326620, (Mouse) 319158, (Mouse) 97122