Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
|Tested species reactivity||Tag|
|Published species reactivity||Yeast, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to the nine amino acid sequence HA-tag (YPYDVPDYA).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunoprecipitation (IP)||5 ug|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. An HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ubiquitin-dependent recruitment of the Bloom syndrome helicase upon replication stress is required to suppress homologous recombination.
71-5500 was used in immunocytochemistry and western blot to study the mechanism through which the Bloom syndrome helicase suppresses homologous recombination at sites of DNA damage
|Tikoo S,Madhavan V,Hussain M,Miller ES,Arora P,Zlatanou A,Modi P,Townsend K,Stewart GS,Sengupta S||The EMBO journal (32:1778)||2013|
|Yeast||Not Cited||Genetic and biochemical analysis of high iron toxicity in yeast: iron toxicity is due to the accumulation of cytosolic iron and occurs under both aerobic and anaerobic conditions.||Lin H,Li L,Jia X,Ward DM,Kaplan J||The Journal of biological chemistry (286:3851)||2011|
|Human||Not Cited||Connexin 43 hemichannels mediate the Ca2+ influx induced by extracellular alkalinization.||Schalper KA,Sánchez HA,Lee SC,Altenberg GA,Nathanson MH,Sáez JC||American journal of physiology. Cell physiology (299:C1504)||2010|
|Mouse||Not Cited||In vivo requirement of the alpha-syntrophin PDZ domain for the sarcolemmal localization of nNOS and aquaporin-4.||Adams ME,Mueller HA,Froehner SC||The Journal of cell biology (155:113)||2001|
||In vivo requirement of the alpha-syntrophin PDZ domain for the sarcolemmal localization of nNOS and aquaporin-4.||Adams ME,Mueller HA,Froehner SC||The Journal of cell biology (155:113)||2001|
|Human||Not Cited||Characterization of multiple isoforms of protein 4.1R expressed during erythroid terminal differentiation.||Gascard P,Lee G,Coulombel L,Auffray I,Lum M,Parra M,Conboy JG,Mohandas N,Chasis JA||Blood (92:4404)||1998|