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HADH Polyclonal Antibody

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HADH Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 19828-1-AP (HADH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

HADH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:400 (under 40x lens).

HADH Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

HADH Antibody in Immunoprecipitation (IP) — IP result of anti-HADH (IP:19828-1-AP, 3ug; Detection:19828-1-AP 1:700) with HepG2 cells lysate 400ug.

Product Details

19828-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 5 publications 5 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HADH Fusion Protein Ag13928 (1-314 aa encoded by BC000306) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.24 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MAFVTRQFM RSVSSSSTAS ASAKKIIVKH VTVIGGGLMG AGIAQVAAAT GHTVVLVDQT EDILAKSKKG IEESLRKVAK KKFAENPKAG DEFVEKTLST IATSTDAASV VHSTDLVVEA IVENLKVKNE LFKRLDKFAA EHTIFASNTS SLQITSIANA TTRQDRFAGL HFFNPVPVMK LVEVIKTPMT SQKTFESLVD FSKALGKHPV SCKDTPGFIV NRLLVPYLME AIRLYERGDA SKEDIDTAMK LGAGYPMGPF ELLDYVGLDT TKFIVDGWHE MDAENPLHQP SPSLNKLVAE NKFGKKTGEG FYKYK (1-314 aa encoded by BC000306)

Target Information

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

High-Intensity Interval Training Improves Markers of Oxidative Metabolism in Skeletal Muscle of Individuals With Obesity and Insulin Resistance.

Frontiers in physiology

de Matos MA,Vieira DV,Pinhal KC,Lopes JF,Dias-Peixoto MF,Pauli JR,de Castro Magalhães F,Little JP,Rocha-Vieira E,Amorim FT

Thu Oct 01 00:00:00 EDT 2020

PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.

EBioMedicine

Lyu Z,Mao Z,Li Q,Xia Y,Liu Y,He Q,Wang Y,Zhao H,Lu Z,Zhou Q

Sat Dec 01 00:00:00 EST 2018

Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.

Scientific reports

Wu L,Guo X,Lyu Y,Clarke SL,Lucas EA,Smith BJ,Hildebrand D,Wang W,Medeiros DM,Shen X,Lin D

Tue Nov 07 00:00:00 EST 2017

Lack of β, β-carotene-9', 10'-oxygenase 2 leads to hepatic mitochondrial dysfunction and cellular oxidative stress in mice.

Molecular nutrition & food research

Wu L,Guo X,Hartson SD,Davis MA,He H,Medeiros DM,Wang W,Clarke SL,Lucas EA,Smith BJ,von Lintig J,Lin D

Mon May 01 00:00:00 EDT 2017

Disruption of the histidine triad nucleotide-binding hint2 gene in mice affects glycemic control and mitochondrial function.

Hepatology (Baltimore, Md.)

Martin J,Maurhofer O,Bellance N,Benard G,Graber F,Hahn D,Galinier A,Hora C,Gupta A,Ferrand G,Hoppeler H,Rossignol R,Dufour JF,St-Pierre MV

Wed May 01 00:00:00 EDT 2013

Disruption of the histidine triad nucleotide-binding hint2 gene in mice affects glycemic control and mitochondrial function.

Hepatology (Baltimore, Md.)

Martin J,Maurhofer O,Bellance N,Benard G,Graber F,Hahn D,Galinier A,Hora C,Gupta A,Ferrand G,Hoppeler H,Rossignol R,Dufour JF,St-Pierre MV

Wed May 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: HCDH; Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; hydroxylacyl-Coenzyme A dehydrogenase short chain; hydroxylacyl-Coenzyme A dehydrogenase, short chain; hydroxylacyl-Coenzyme A dehydrogenase-dehydrogenase; L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain; M/SCHAD; medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; MGC8392; short chain 3-hydroxyacyl-CoA dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; testis secretory sperm-binding protein Li 203a

Gene Aliases: AA409008; AU019341; AW742602; HAD; HAD1; HADH; HADH1; HADHSC; HCDH; HHF4; MSCHAD; SCHAD

UniProt ID: (Human) Q16836, (Mouse) Q61425, (Rat) Q9WVK7

Entrez Gene ID: (Human) 3033, (Mouse) 15107, (Rat) 113965

Function(s)

fatty acid beta-oxidation response to activity response to insulin response to drug negative regulation of insulin secretion lipid metabolic process fatty acid metabolic process oxidation-reduction process response to hormone

Process(es)

3-hydroxyacyl-CoA dehydrogenase activity NAD+ binding oxidoreductase activity