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|Tested species reactivity||Human, Mouse, Rabbit, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, BSA conjugated, correspondiong to the residues K(168) A D G G R E S K R K R E L(181) of to human HAND1.|
|Storage buffer||PBS with 50% ammonium sulfate|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20319 detects HAND1 in mouse and rat samples. Antibody is expected to cross-react with human (100%) and rabbit (92%) based on sequence homology.
PA1-20319 has been successfully used in immunohistochemistry and Western blot procedures.
Positive control: Embryonic mouse brain.
The PA1-20319 immunogen is a synthetic peptide, BSA conjugated, correspondiong to the residues K(168) A D G G R E S K R K R E L(181) of to human HAND1.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
HAND1 is a transcription regulator and plays an essential role in early trophoblast differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. HAND1 binds the DNA sequence 5'-NRTCTG-3'.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
bHLHa27; class A basic helix-loop-helix protein 27; eHAND; eHAND basic helix-loop-helix transcription factor; Ehand1; extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1; heart and neural crest derivatives expressed transcript 1; Heart- and neural crest derivatives-expressed protein 1; helix-loop-helix transcription factor expressed in extraembryonic mesoderm and trophoblast; Hxt; RP23-268K22.4; Th1; thing-1; Thing1
bHLHa27; eHAND; Ehand1; Hand1; Hxt; Th1; Thing1