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Invitrogen
HAND2 Polyclonal Antibody
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FIGURE: 1 / 3
HAND2 Antibody (PA5-115339) in ICC/IF
Immunocytochemistry analysis of HAND2 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115339). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-115339
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HAND2(Accession P61296), corresponding to amino acid residues G45-P95.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total HAND2.
Target Information
The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: basic helix-loop-helix transcription factor HAND2; bHLHa26; Class A basic helix-loop-helix protein 26; Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2; dHAND; dHand protein; heart and neural crest derivatives expressed transcript 2; Heart- and neural crest derivatives-expressed protein 2; Helix-loop-helix transcription factor expressed in embryo and deciduum; Thing-2
Gene Aliases: AI225906; AI661148; BHLHA26; DHAND; DHAND2; Ehand2; HAND2; Hed; Th2; Thing2
UniProt ID: (Human) P61296, (Rat) P61295, (Mouse) Q61039
Entrez Gene ID: (Human) 9464, (Rat) 64637, (Mouse) 15111
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
AT DNA binding
transcription coactivator activity
protein binding
transcription factor binding
activating transcription factor binding
protein homodimerization activity
transcription regulatory region DNA binding
protein heterodimerization activity
E-box binding
DNA binding
sequence-specific DNA binding
protein dimerization activity
basic helix-loop-helix transcription factor
angiogenesis
in utero embryonic development
heart looping
suckling behavior
cardiac right ventricle formation
cardiac neural crest cell migration involved in outflow tract morphogenesis
regulation of secondary heart field cardioblast proliferation
apoptotic process involved in heart morphogenesis
noradrenergic neuron differentiation
transcription from RNA polymerase II promoter
heart development
adult heart development
mesenchymal cell proliferation
positive regulation of cardiac muscle hypertrophy
positive regulation of gene expression
negative regulation of gene expression
negative regulation of cardiac muscle cell apoptotic process
regulation of tissue remodeling
odontogenesis of dentin-containing tooth
embryonic digit morphogenesis
negative regulation of DNA binding
negative regulation of sequence-specific DNA binding transcription factor activity
tongue development
negative regulation of osteoblast differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
sympathetic nervous system development
thymus development
peripheral nervous system neuron development
palate development
cartilage morphogenesis
coronary artery morphogenesis
visceral serous pericardium development
cardiac neural crest cell development involved in outflow tract morphogenesis
cell proliferation involved in outflow tract morphogenesis
positive regulation of ERK1 and ERK2 cascade
positive regulation of p38MAPK cascade
positive regulation of transcription regulatory region DNA binding
positive regulation of transcription from RNA polymerase II promoter involved in norepinephrine biosynthetic process
positive regulation of semaphorin-plexin signaling pathway involved in outflow tract morphogenesis
heart morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
neural crest cell development
cell differentiation
negative regulation of apoptotic process
mesenchyme development
determination of heart left/right asymmetry
cellular response to retinoic acid
cellular response to organic cyclic compound
multicellular organism development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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