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Invitrogen
HARS2 Polyclonal Antibody
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FIGURE: 1 / 1
HARS2 Antibody (PA5-96805) in WB
Western blot analysis of HARS2 in extracts of various cell lines. Samples were incubated with HARS2 Polyclonal antibody (Product # PA5-96805) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-96805
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 217-506 of human HARS2 (NP_0363401)
Conjugate
Form
Liquid
Concentration
9.51 mg/mL
Amount
951 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: IVDGMFAVCG VPESKFRAIC SSIDKLDKMA WKDVRHEMVV KKGLAPEVAD RIGDYVQCHG GVSLVEQMFQ DPRLSQNKQA LEGLGDLKLL FEYLTLFGIA DKISFDLSLA RGLDYYTGVI YEAVLLQTPT QAGEEPLNVG SVAAGGRYDG LVGMFDPKGH KVPCVGLSIG VERIFYIVEQ RMKTKGEKVR TTETQVFVAT PQKNFLQERL KLIAELWDSG IKAEMLYKNN PKLLTQLHYC ESTGIPLVVI IGEQELKEGV IKIRSVASRE EVAIKRENFV AEIQKRLSES; Positive Samples: U-251MG, Rat liver; Cellular Location: Mitochondrion matrix
Target Information
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 6.1.1.21; HARS-related; HisRS; histidine translase; histidine tRNA ligase 2, mitochondrial (putative); Histidine--tRNA ligase, mitochondrial; Histidine--tRNA ligase-like; histidine-tRNA ligase homolog; Histidyl-tRNA synthetase; histidyl-tRNA synthetase 2, mitochondrial (putative); histidyl-tRNA synthetase 2, mitochondrial, hisRS; probable histidine--tRNA ligase, mitochondrial; probable histidyl-tRNA synthetase, mitochondrial; unnamed protein product; zinc finger, matrin type 2
Gene Aliases: 4631412B19Rik; HARS2; Hars2l; HARSL; HARSR; HisRS; HO3; PRLTS2; RGD1308426; Zmat2
UniProt ID: (Human) P49590, (Mouse) Q99KK9
Entrez Gene ID: (Human) 23438, (Rat) 307491, (Mouse) 70791
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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