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Invitrogen
HAS2 Polyclonal Antibody
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FIGURE: 1 / 7
HAS2 Antibody (PA5-115388) in ICC/IF
Immunocytochemistry analysis of HAS2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115388) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115388
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HAS2(Accession Q92819), corresponding to amino acid residues L518-V552.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Hyaluronan synthase 2.
Target Information
Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HA synthase 2; Hyaluronan synthase 2; hyaluronan synthase homolog; Hyaluronate synthase 2; Hyaluronic acid synthase 2
Gene Aliases: HAS2
UniProt ID: (Human) Q92819, (Mouse) P70312, (Rat) O35776
Entrez Gene ID: (Human) 3037, (Mouse) 15117, (Rat) 25694
vasculogenesis
kidney development
positive regulation of cell proliferation
positive regulation of keratinocyte proliferation
positive regulation of smooth muscle cell migration
hyaluronan biosynthetic process
positive regulation of cell migration
positive regulation of urine volume
cellular response to platelet-derived growth factor stimulus
atrioventricular canal development
estrous cycle
extracellular polysaccharide biosynthetic process
positive regulation of keratinocyte migration
bone morphogenesis
renal water absorption
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to fluid shear stress
extracellular matrix assembly
endocardial cushion to mesenchymal transition
positive regulation of substrate adhesion-dependent cell spreading
positive regulation of hyaluronan biosynthetic process
positive regulation of monocyte aggregation
cell adhesion
hyaluronan metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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