|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide EESFQELVEDYRRVIERLAQE with N-terminal added lysine conjugated to KLH, corresponding to amino acids 399-419 of Human HAT1.The corresponding sequence differs by three amino acids in chicken and by one amino acid in mouse (putative sequence).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-25446 detects HAT1 from human, mouse samples.
PA1-25446 has been successfully used in Western blot procedures, where a band of approximately 46 kDa is detected. Positive controls: 293 cell extracts, NIH-3T3 cell extracts, and chicken fibroblasts.
The PA1-25446 immunogen is a synthetic peptide EESFQELVEDYRRVIERLAQE with N-terminal added lysine conjugated to KLH, corresponding to amino acids 399-419 of Human HAT1.The corresponding sequence differs by three amino acids in chicken and by one amino acid in mouse (putative sequence).
The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Subcellular proteomics reveals a role for nucleo-cytoplasmic trafficking at the DNA replication origin activation checkpoint.
PA1-25446 was used in western blot to study the role of nucleocytoplasmic trafficing in DNA replication origin checkpoint activation as revealed by quantitative SILAC analysis
|Mulvey CM,Tudzarova S,Crawford M,Williams GH,Stoeber K,Godovac-Zimmermann J||Journal of proteome research (12:1436)||2013|