Immunofluorescent analysis of HAUS1 showing staining in the centrosome of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a HAUS1 polyclonal antibody (Product # PA5-34874) diluted at 1:1000. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 278 of HAUS1(CCDC5 ) (Uniprot ID#Q96CS2)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34874 targets HAUS1 in WB applications and shows reactivity with Human samples.
The PA5-34874 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 278 of HAUS1(CCDC5 ) (Uniprot ID#Q96CS2).
Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.