Immunofluorescent analysis of HAUS6 in U2OS cells using a HAUS6 polyclonal antibody (Product # PA5-31257) at a dilution of 1:500 (red). HAUS6 co-localizes at the mitotic spindle with Hice1-GFP (HAUS-GFP, green). DAPI staining (blue) shows mitotic chromosomes. Arrows indicate spindle poles.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 552 and 955 of Human HAUS6|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31257 targets HAUS6 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-31257 immunogen is recombinant fragment corresponding to a region within amino acids 552 and 955 of Human HAUS6.
HAUS6 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb and apos;augmentare, and apos; meaning and apos;to increase. and apos; The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.