PA1-917 detects the HCN1 from rat samples.
PA1-917 has been successfully used in Western blot procedures. By Western blot, this antibody detects bands at ~98 kDa and ~115 kDa representing the non-glycosylated and glycosylated form of HCN1 in rat brain extract.
The PA1-917 immunizing peptide corresponds to amino acid residues 875-890 from human HCN1.
HCN1 includes Hyperpolarization-activated cation channels of the HCN gene family, which contribute to spontaneous rhythmic activity in both heart and brain. HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. The HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.
Protein Aliases: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Gene Aliases: Hcn1
UniProt ID: (Rat) Q9JKB0
Entrez Gene ID: (Rat) 84390