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|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: L(875) N T D P D A E K P R F A N S L(890)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-917 detects the HCN1 from rat samples.
PA1-917 has been successfully used in Western blot procedures. By Western blot, this antibody detects bands at ~98 kDa and ~115 kDa representing the non-glycosylated and glycosylated form of HCN1 in rat brain extract.
The PA1-917 immunizing peptide corresponds to amino acid residues 875-890 from human HCN1.
Hyperpolarization-activated cation channels of the HCN gene family such as HCN1, contribute to spontaneous rhythmic activity in both heart and brain. HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells; as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations.
The HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel-1; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1