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To determine the titer, an ELISA was performed using anti-HDAC1 crude serum, purified anti-HDAC1 antibodies (Cat. no. 49-1025), and the column flow through obtained from the antibody purification step. The antigen used was the C-terminal peptide used for immunization. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,250.
|Tested species reactivity||Mouse , Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||raised in rabbits against the C-terminal region of human HDAC1, using a KLH-conjugated synthetic peptide.|
|Contains||<0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||2.4 ug|
|ELISA (ELISA)||1:100 to 1:300|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p. HDAC1 is a class I histone deacetylase containing 482 amino acid residues. HDAC1 has been shown to interact directly with transcription factors and has been shown to deacetylate histone proteins H3 and H4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Transcriptional repression of ER through hMAPK dependent histone deacetylation by class I HDACs.
49-1025 was used in western blot to study the relationship between hyperactivation of MAPK and the estrogen receptor in breast cancer cells
|Plotkin A,Volmar CH,Wahlestedt C,Ayad N,El-Ashry D||Breast cancer research and treatment (147:249)||2014|
reduced potassium dependency, yeast homolog-like 1, histone deacetylase 1, HDAC1, RPD3L1
GON-10, HD1, RPD3L1, MommeD5, RPD3, Hdac1-ps
Nucleoside, nucleotide and nucleic acid metabolism mRNA transcription mRNA transcription regulation Chromatin packaging and remodeling Protein metabolism and modification Protein modification Cell cycle Cell cycle control