Immunofluorescent analysis of HDAC1 (green) in 3T3 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a HDAC1 rabbit polyclonal antibody (Product # PA1-860), at a concentration of 10ug/ml in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-rabbit IgG Superclonal secondary antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:1000 for 30 minutes at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Dog, Hamster, Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay dependent|
|Immunocytochemistry (ICC)||2.0 µg/mL|
|Immunofluorescence (IF)||10 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||0.2 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Western Blot (WB)||See 20 publications below|
|ChIP assay (ChIP)||See 4 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Immunoprecipitation (IP)||See 3 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
PA1-860 detects histone deacetylase 1 (HDAC1) from human, mouse, rat, hamster and canine tissues and cells.
PA1-860 has been successfully used in Western blot, immunoprecipitation, ChIP, immunofluoresence, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~62 kDa protein representing HDAC1 from HeLa cell lysate. Immunocytochemical staining of HDAC1 in HeLa cells with PA1-860 results in nuclear staining.
The PA1-860 immunogen is a synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1. This immunizing peptide (Cat. # PEP-069) is available for use in neutralization and control experiments.
Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p. HDAC1 is a class I histone deacetylase containing 482 amino acid residues. HDAC1 has been shown to interact directly with transcription factors and has been shown to deacetylate histone proteins H3 and H4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Lactate's effect on human neuroblastoma cell bioenergetic fluxes.
PA1-860 was used in western blot to investigate how lactate affects neuron metabolism
|E L,Swerdlow RH||Biochemical pharmacology (99:88)||2016|
Combined HDAC1 and HDAC2 Depletion Promotes Retinal Ganglion Cell Survival After Injury Through Reduction of p53 Target Gene Expression.
PA1-860 was used in immunohistochemistry - paraffin section to determine the functional role of HDAC1 and HDAC2 in an acute model of optic nerve injury.
|Lebrun-Julien F,Suter U||ASN neuro (7:null)||2015|
HDAC1 and HDAC2 collectively regulate intestinal stem cell homeostasis.
PA1-860 was used in western blot to investigate the role of HDAC1 and HDAC2 in intestinal homeostasis
|Zimberlin CD,Lancini C,Sno R,Rosekrans SL,McLean CM,Vlaming H,van den Brink GR,Bots M,Medema JP,Dannenberg JH||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (29:2070)||2015|
Specificity in circadian clock feedback from targeted reconstitution of the NuRD corepressor.
PA1-860 was used in western blot to investigate how different NuRD subunits regulate the PER complex.
|Kim JY,Kwak PB,Weitz CJ||Molecular cell (56:738)||2014|
Effect of high-intensity exercise on aged mouse brain mitochondria, neurogenesis, and inflammation.
PA1-860 was used in western blot to determine how intensive exercise affects brain bioenergetics, inflammation, and neurogenesis-relevant parameters
|E L,Burns JM,Swerdlow RH||Neurobiology of aging (35:2574)||2014|
Identification of myelin transcription factor 1 (MyT1) as a subunit of the neural cell type-specific lysine-specific demethylase 1 (LSD1) complex.
PA1-860 was used in western blot to study the role of MyT1 as a component of an LSD-1 complex specific to neural cells
|Yokoyama A,Igarashi K,Sato T,Takagi K,Otsuka I M,Shishido Y,Baba T,Ito R,Kanno J,Ohkawa Y,Morohashi K,Sugawara A||The Journal of biological chemistry (289:18152)||2014|
Unfolded protein response signaling by transcription factor XBP-1 regulates ADAM10 and is affected in Alzheimer's disease.
PA1-860 was used in western blot to study the regulation of ADAM10 expression by the unfolded protein response transcription factor XBP-1 and the implications for Alzheimer's disease
|Reinhardt S,Schuck F,Grösgen S,Riemenschneider M,Hartmann T,Postina R,Grimm M,Endres K||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (28:978)||2014|
Overlapping chromatin-remodeling systems collaborate genome wide at dynamic chromatin transitions.
PA1-860 was used in western blot to study the roles of Brg1, Chd4 and Snf2h in genome-wide remodeling of chromatin
|Morris SA,Baek S,Sung MH,John S,Wiench M,Johnson TA,Schiltz RL,Hager GL||Nature structural and molecular biology (21:73)||2014|
SIRT1 collaborates with ATM and HDAC1 to maintain genomic stability in neurons.
PA1-860 was used in western blot to study the roles of SIRT1, ATM and HDAC1 in the DNA double-strand break response of neurons and the therapeutic significance for neurodegenerative disease
|Dobbin MM,Madabhushi R,Pan L,Chen Y,Kim D,Gao J,Ahanonu B,Pao PC,Qiu Y,Zhao Y,Tsai LH||Nature neuroscience (16:1008)||2013|
Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program.
PA1-860 was used in western blot to study the mechanism by which SFMBT1 modulates epigenetic silencing and mitogenic gene expression
|Lin S,Shen H,Li JL,Tang S,Gu Y,Chen Z,Hu C,Rice JC,Lu J,Wu L||The Journal of biological chemistry (288:6238)||2013|
Altered LKB1/CREB-regulated transcription co-activator (CRTC) signaling axis promotes esophageal cancer cell migration and invasion.
PA1-860 was used in western blot to study the role of LKB1/CREB-regulated transcription co-activator signaling in esophageal cancer cell migration and invasion
|Gu Y,Lin S,Li JL,Nakagawa H,Chen Z,Jin B,Tian L,Ucar DA,Shen H,Lu J,Hochwald SN,Kaye FJ,Wu L||Oncogene (31:469)||2012|
Differential response of cancer cells to HDAC inhibitors trichostatin A and depsipeptide.
PA1-860 was used in western blot to investigate the effects of trichostatin A and depsipeptide on cancer cells
|Chang J,Varghese DS,Gillam MC,Peyton M,Modi B,Schiltz RL,Girard L,Martinez ED||British journal of cancer (106:116)||2012|
Histone Deacetylase Inhibitors and Mithramycin A Impact a Similar Neuroprotective Pathway at a Crossroad between Cancer and Neurodegeneration.
PA1-860 was used in western blot to study the role of histone deacetylase pathways in neurodegeneration and oncogenic transformation
|Sleiman SF,Berlin J,Basso M,Karuppagounder SS,Rohr J,Ratan RR||Pharmaceuticals (Basel, Switzerland) (4:1183)||2011|
Identification of four potential epigenetic modulators from the NCI structural diversity library using a cell-based assay.
PA1-860 was used in western blot to investigate potential epigenetic modulators
|Best AM,Chang J,Dull AB,Beutler JA,Martinez ED||Journal of biomedicine and biotechnology (2011:null)||2011|
Selective association of peroxiredoxin 1 with genomic DNA and COX-2 upstream promoter elements in estrogen receptor negative breast cancer cells.
PA1-860 was used in western blot to investigate the role of peroxiredoxin 1 in the pathogenesis of estrogen receptor negative breast cancer
|Wang X,He S,Sun JM,Delcuve GP,Davie JR||Molecular biology of the cell (21:2987)||2010|
EGF regulates survivin stability through the Raf-1/ERK pathway in insulin-secreting pancreatic ß-cells.
PA1-860 was used in western blot to investigate the regulation of survivin stability by EGF
|Wang H,Gambosova K,Cooper ZA,Holloway MP,Kassai A,Izquierdo D,Cleveland K,Boney CM,Altura RA||BMC molecular biology (11:null)||2010|
Gfi1 coordinates epigenetic repression of p21Cip/WAF1 by recruitment of histone lysine methyltransferase G9a and histone deacetylase 1.
PA1-860 was used in western blot to study the role of growth factor independent 1 Gfi1 in epigenetic repression of p21Cip/WAF1.
|Duan Z,Zarebski A,Montoya-Durango D,Grimes HL,Horwitz M||Molecular and cellular biology (25:10338)||2005|
Transcriptional repression of peroxisome proliferator-activated receptor beta/delta in murine keratinocytes by CCAAT/enhancer-binding proteins.
PA1-860 was used in western blot to study the relationship between PPARbeta and C/EBP transcription factors and its effect on cell proliferation and differentiation.
|Di-Poï N,Desvergne B,Michalik L,Wahli W||The Journal of biological chemistry (280:38700)||2005|
Nucleolar localization of hepatic c-Myc: a potential mechanism for c-Myc regulation.
PA1-860 was used in western blot to investigate the localization of hepatic c-Myc in the nucleolus and its functional significance
|Sanders JA,Gruppuso PA||Biochimica et biophysica acta (1743:141)||2005|
Mitogen- and stress-activated protein kinase 1 activity and histone h3 phosphorylation in oncogene-transformed mouse fibroblasts.
PA1-860 was used in western blot to study the effect of mitogen- and stress-activated protein kinase 1 on histone H3 phosphorylation.
|Drobic B,Espino PS,Davie JR||Cancer research (64:9076)||2004|
Modification of de novo DNA methyltransferase 3a (Dnmt3a) by SUMO-1 modulates its interaction with histone deacetylases (HDACs) and its capacity to repress transcription.
PA1-860 was used in western blot to study the regulation of DNA methyltransferase Dnmt3a enzymatic activity and transcriptional regulation.
|Ling Y,Sankpal UT,Robertson AK,McNally JG,Karpova T,Robertson KD||Nucleic acids research (32:598)||2004|
Pf1, a novel PHD zinc finger protein that links the TLE corepressor to the mSin3A-histone deacetylase complex.
PA1-860 was used in western blot to investigate the role of Pf1 in the two global transcription networks, mSin3A and TLE.
|Yochum GS,Ayer DE||Molecular and cellular biology (21:4110)||2001|
HDAC1 and HDAC2 control the specification of neural crest cells into peripheral glia.
PA1-860 was used in ChIP assay, immunohistochemistry, and western blot to study the roles of Pax3, Sox10 and Fabp7 in the mechanism by which HDAC-1 and -2 control the specification of neural crest cells into peripheral glia
|Jacob C,Lötscher P,Engler S,Baggiolini A,Varum Tavares S,Brügger V,John N,Büchmann-Møller S,Snider PL,Conway SJ,Yamaguchi T,Matthias P,Sommer L,Mantei N,Suter U||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:6112)||2014|
Chromatin modifications induced by the AML1-ETO fusion protein reversibly silence its genomic targets through AML1 and Sp1 binding motifs.
PA1-860 was used in ChIP assay to investigate the importance of Sp1 in functional chromatin modifications induced by AML1-ETO
|Maiques-Diaz A,Chou FS,Wunderlich M,Gómez-López G,Jacinto FV,Rodriguez-Perales S,Larrayoz MJ,Calasanz MJ,Mulloy JC,Cigudosa JC,Alvarez S||Leukemia (26:1329)||2012|
Tax relieves transcriptional repression by promoting histone deacetylase 1 release from the human T-cell leukemia virus type 1 long terminal repeat.
PA1-860 was used in chromatin immunoprecipitation to study the effect of HTLV-1 viral transcriptional activator Tax on transcriptional regulation.
|Lu H,Pise-Masison CA,Linton R,Park HU,Schiltz RL,Sartorelli V,Brady JN||Journal of virology (78:6735)||2004|
Retinoblastoma protein transcriptional repression through histone deacetylation of a single nucleosome.
PA1-860 was used in chromatin immunoprecipitation to investigate the mechanism of the retinoblastoma protein transcriptional repression.
|Morrison AJ,Sardet C,Herrera RE||Molecular and cellular biology (22:856)||2002|
Lactate administration reproduces specific brain and liver exercise-related changes.
PA1-860 was used in immunohistochemistry to study the ability of exogenous lactate to recapitulate some of the liver and brain changes associated with exercise
|E L,Lu J,Selfridge JE,Burns JM,Swerdlow RH||Journal of neurochemistry (127:91)||2013|
Retrograde GABA signaling adjusts sound localization by balancing excitation and inhibition in the brainstem.
PA1-860 was used in immunohistochemistry to study the role of retrograde GABA signaling in adjusting sound localization
|Magnusson AK,Park TJ,Pecka M,Grothe B,Koch U||Neuron (59:125)||2008|
Protein kinase CK2 regulates the dimerization of histone deacetylase 1 (HDAC1) and HDAC2 during mitosis.
PA1-860 was used in immunocytochemistry, immunoprecipitation, and western blot to study the modulation of the dimerization of HDAC-1 and -2 by CK2-mediated phosphorylation during mitosis
|Khan DH,He S,Yu J,Winter S,Cao W,Seiser C,Davie JR||The Journal of biological chemistry (288:16518)||2013|
Stress-induced epigenetic transcriptional memory of acetylcholinesterase by HDAC4.
PA1-860 was used in immunoprecipitation to study the role of HDAC4 in mediating stress-associated epigenetic changes in acetylcholinesterase transcription and splicing
|Sailaja BS,Cohen-Carmon D,Zimmerman G,Soreq H,Meshorer E||Proceedings of the National Academy of Sciences of the United States of America (109:E3687)||2012|
Differential modulation of the oligodendrocyte transcriptome by sonic hedgehog and bone morphogenetic protein 4 via opposing effects on histone acetylation.
PA1-860 was used in immunoprecipitation to study the role of histone acetylation in the different effects on the oligodendrocyte transcriptome of sonic hedgehog and BMP4
|Wu M,Hernandez M,Shen S,Sabo JK,Kelkar D,Wang J,O'Leary R,Phillips GR,Cate HS,Casaccia P||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:6651)||2012|
Differential intranuclear organization of transcription factors Sp1 and Sp3.
PA1-860 was used in immunocytochemistry to study the nuclear localization of transcription factors Sp1 and Sp3.
|He S,Sun JM,Li L,Davie JR||Molecular biology of the cell (16:4073)||2005|