Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil or breast carcinoma.
Mammalian HADC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. The deacetylation of histones is associated with transcriptional silencing.
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Protein Aliases: GON-10; HD1; Histone deacetylase 1; reduced potassium dependency; reduced potassium dependency, yeast homolog-like 1; RPD3; RPD3L1; yeast homolog-like 1
Gene Aliases: GON-10; HD1; HDAC1; RPD3; RPD3L1
UniProt ID: (Human) Q13547
Entrez Gene ID: (Human) 3065