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Invitrogen

HDAC2 Polyclonal Antibody

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10 Published Figures
16 References
View all (33) HDAC2 antibodies

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HDAC2 Polyclonal Antibody

Product Details

PA1-861

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5 µg/mL
View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

0.4 µg/mL
-

Immunocytochemistry (ICC/IF)

1.0 µg/mL
View 1 publication 1 publication

Flow Cytometry (Flow)

1-20 µg/mL
-

Immunoprecipitation (IP)

Assay-dependent
View 1 publication 1 publication

ChIP assay (ChIP)

1-3 µL
View 2 publications 2 publications
Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2118520

Product Specific Information

PA1-861 detects histone deacetylase 2 (HDAC2) from canine, hamster, human, mouse, non-human primate, rat tissues and cells.

PA1-861 has been successfully used in Western blot, ChIP, immunoprecipitation, immunofluorescence, flow cytometry, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing HDAC2 and a ~40 kDa protein of unknown identity from HeLa cell lysate. Immunocytochemical staining of HDAC2 in HeLa cells with PA1-861 results in nuclear staining.

The PA1-861 immunogen is a synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2. This immunizing peptide (Cat. # PEP-070) is available for use in neutralization and control experiments.

Target Information

HDAC2 belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HD2; hdac 2; hdac-2; Histone deacetylase 2; histone deacetylase 2-like protein; Protein deacylase HDAC2; transcriptional regulator homolog RPD3; unnamed protein product; YY1 transcription factor-binding protein; YY1-associated factor 1

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Gene Aliases: D10Wsu179e; HD2; HDAC2; I79_010219; KDAC2; mRPD3; RPD3; YAF1; Yy1bp

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UniProt ID: (Human) Q92769, (Mouse) P70288

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Entrez Gene ID: (Dog) 475035, (Human) 3066, (Mouse) 15182, (Rat) 84577, (Chinese hamster) 100764557

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Function(s)
transcription cofactor binding chromatin binding RNA binding histone deacetylase activity protein binding hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides deacetylase activity enzyme binding heat shock protein binding nucleosomal DNA binding protein deacetylase activity histone binding histone deacetylase binding NF-kappaB binding RNA polymerase II sequence-specific DNA binding transcription factor binding DNA-binding transcription factor binding histone deacetylase activity, hydrolytic mechanism protein decrotonylase activity histone decrotonylase activity protein de-2-hydroxyisobutyrylase activity protein lysine delactylase activity promoter-specific chromatin binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding core promoter binding RNA polymerase II repressing transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor binding histone deacetylase activity (H3-K14 specific) chromatin DNA binding NAD-dependent histone deacetylase activity (H3-K14 specific) histone deacetylase activity (H3-K9 specific) histone deacetylase activity (H4-K16 specific) Krueppel-associated box domain binding sequence-specific DNA binding poly(A) RNA binding NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K18 specific)
Process(es)
negative regulation of transcription from RNA polymerase II promoter maintenance of chromatin silencing transcription, DNA-templated regulation of transcription, DNA-templated protein deacetylation positive regulation of cell proliferation epidermal cell differentiation positive regulation of epithelial to mesenchymal transition positive regulation of receptor biosynthetic process negative regulation of neuron projection development dendrite development chromatin modification histone deacetylation hippocampus development neuron differentiation positive regulation of interleukin-1 production positive regulation of tumor necrosis factor production circadian regulation of gene expression odontogenesis of dentin-containing tooth positive regulation of tyrosine phosphorylation of Stat3 protein embryonic digit morphogenesis ATP-dependent chromatin remodeling negative regulation of apoptotic process negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of proteolysis negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process positive regulation of oligodendrocyte differentiation positive regulation of sequence-specific DNA binding transcription factor activity regulation of protein kinase B signaling cardiac muscle cell development negative regulation of cardiac muscle cell proliferation regulation of sarcomere organization hair follicle placode formation negative regulation of dendritic spine development eyelid development in camera-type eye fungiform papilla formation cellular response to hydrogen peroxide histone H3 deacetylation histone H4 deacetylation negative regulation of canonical Wnt signaling pathway regulation of protein deacetylation negative regulation of peptidyl-lysine acetylation negative regulation of intrinsic apoptotic signaling pathway response to amphetamine cardiac muscle hypertrophy response to caffeine response to lipopolysaccharide cellular response to heat response to nicotine response to cocaine response to drug behavioral response to ethanol response to hyperoxia histone H3-K9 modification cellular response to retinoic acid cellular response to transforming growth factor beta stimulus cellular response to dopamine chromatin organization chromatin remodeling response to xenobiotic stimulus negative regulation of transcription by competitive promoter binding negative regulation of cell migration negative regulation of transforming growth factor beta receptor signaling pathway heterochromatin assembly positive regulation of intracellular estrogen receptor signaling pathway protein modification process regulation of cell fate specification positive regulation of transcription, DNA-templated progesterone receptor signaling pathway response to alcohol positive regulation of male mating behavior negative regulation of stem cell population maintenance positive regulation of stem cell population maintenance response to amyloid-beta regulation of stem cell differentiation
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