Invitrogen

HDAC2 Polyclonal Antibody, Alexa Fluor™ 555

View all (39) HDAC2 antibodies

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HDAC2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HDAC2 (orange) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a HDAC2 Polyclonal Antibody, AlexaFluor 555 conjugate (Product # PA1-861-A555) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Product Details

PA1-861-A555

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	1-10 µg/mL	-

Product Specifications
Species Reactivity	Dog, Hamster, Human, Mouse, Non-human primate, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2 View antigen
Conjugate	Alexa Fluor™ 555
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2664285

Product Specific Information

PA1861A555 detects histone deacetylase 2 (HDAC2) from canine, hamster, human, mouse, non-human primate, rat tissues and cells.

The PA1861A555 immunogen is a synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2. This immunizing peptide (Cat. # PEP-070) is available for use in neutralization and control experiments.

Target Information

HDAC2 belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HD2; hdac 2; hdac-2; Histone deacetylase 2; histone deacetylase 2-like protein; transcriptional regulator homolog RPD3; YY1 transcription factor-binding protein; YY1-associated factor 1

Gene Aliases: D10Wsu179e; HD2; HDAC2; I79_010219; mRPD3; RPD3; YAF1; Yy1bp

UniProt ID: (Human) Q92769, (Mouse) P70288

Entrez Gene ID: (Human) 3066, (Dog) 475035, (Mouse) 15182, (Rat) 84577, (Chinese hamster) 100764557

negative regulation of transcription from RNA polymerase II promoter response to amphetamine cardiac muscle hypertrophy chromatin remodeling maintenance of chromatin silencing transcription, DNA-templated blood coagulation positive regulation of cell proliferation epidermal cell differentiation positive regulation of epithelial to mesenchymal transition positive regulation of receptor biosynthetic process negative regulation of neuron projection development dendrite development histone deacetylation response to caffeine response to lipopolysaccharide positive regulation of interleukin-1 production positive regulation of tumor necrosis factor production circadian regulation of gene expression positive regulation of collagen biosynthetic process cellular response to heat response to nicotine response to cocaine odontogenesis of dentin-containing tooth response to drug positive regulation of tyrosine phosphorylation of Stat3 protein embryonic digit morphogenesis ATP-dependent chromatin remodeling negative regulation of apoptotic process negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of MHC class II biosynthetic process positive regulation of proteolysis negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter behavioral response to ethanol positive regulation of oligodendrocyte differentiation response to hyperoxia hair follicle placode formation negative regulation of dendritic spine development eyelid development in camera-type eye fungiform papilla formation cellular response to hydrogen peroxide histone H3 deacetylation histone H4 deacetylation cellular response to retinoic acid cellular response to transforming growth factor beta stimulus regulation of signal transduction by p53 class mediator cellular response to dopamine negative regulation of peptidyl-lysine acetylation regulation of transcription, DNA-templated protein deacetylation chromatin modification hippocampus development neuron differentiation rhythmic process positive regulation of sequence-specific DNA binding transcription factor activity regulation of protein kinase B signaling cardiac muscle cell development negative regulation of cardiac muscle cell proliferation regulation of sarcomere organization negative regulation of canonical Wnt signaling pathway regulation of protein deacetylation negative regulation of intrinsic apoptotic signaling pathway histone H3-K9 modification

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding core promoter binding RNA polymerase II repressing transcription factor binding chromatin binding histone deacetylase activity protein binding transcription factor binding deacetylase activity enzyme binding heat shock protein binding nucleosomal DNA binding NAD-dependent histone deacetylase activity (H3-K14 specific) protein deacetylase activity sequence-specific DNA binding poly(A) RNA binding NF-kappaB binding transcription factor activity, sequence-specific DNA binding hydrolase activity histone deacetylase activity (H3-K14 specific) chromatin DNA binding histone deacetylase activity (H3-K9 specific) histone deacetylase activity (H4-K16 specific) Krueppel-associated box domain binding NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K18 specific)