Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
Histone acetylation is a dynamic process whose levels are determined by the net activities of histone acetyltransferases (HATs) and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.
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Protein Aliases: HD2; Histone deacetylase 2; RPD3; transcriptional regulator homolog RPD3; YAF1; YY1-associated factor 1
Gene Aliases: HD2; HDAC2; RPD3; YAF1
UniProt ID: (Human) Q92769
Entrez Gene ID: (Human) 3066