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Invitrogen
HDAC3 Polyclonal Antibody
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FIGURE: 1 / 6
HDAC3 Antibody (PA1-862) in ICC/IF
Immunofluorescent analysis of HDAC3 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a HDAC3 polyclonal antibody (Product # PA1-862) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). HDAC3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-862
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Hamster,
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C D(415) G D H D N D K E S D V E I(428) of human HDAC3.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-862 detects histone deacetylase 3 (HDAC3) from canine, hamster, human and rat tissues and cells.
PA1-862 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~48 kDa protein representing HDAC3 from HeLa cell lysate.
The PA1-862 immunogen is a synthetic peptide corresponding to residues C D(415) G D H D N D K E S D V E I(428) of human HDAC3. This immunizing peptide (Cat. # PEP-071) is available for use in neutralization and control experiments.
Target Information
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HD3; HDAC3; histone deacetylase 3; histone deacetylase 3-like protein; protein deacetylase HDAC3; protein deacylase HDAC3; SMAP45
Gene Aliases: HD3; KDAC3; RPD3; RPD3-2
UniProt ID: (Human) O15379, (Rat) Q6P6W3
Entrez Gene ID: (Dog) 478040, (Human) 8841, (Rat) 84578, (Chinese hamster) 100752639
transcription corepressor binding
DNA binding
chromatin binding
transcription corepressor activity
histone deacetylase activity
protein binding
enzyme binding
cyclin binding
chromatin DNA binding
protein deacetylase activity
histone deacetylase binding
NF-kappaB binding
histone isonicotinyllysine deisonicotinylase activity
DNA-binding transcription factor binding
histone deacetylase activity, hydrolytic mechanism
protein decrotonylase activity
histone decrotonylase activity
protein de-2-hydroxyisobutyrylase activity
protein lysine delactylase activity
deacetylase activity
GTPase binding
ubiquitin-specific protease binding
negative regulation of transcription from RNA polymerase II promoter
establishment of mitotic spindle orientation
in utero embryonic development
positive regulation of protein phosphorylation
protein deacetylation
regulation of mitotic cell cycle
epidermis development
response to xenobiotic stimulus
gene expression
negative regulation of myotube differentiation
positive regulation of protein ubiquitination
regulation of protein stability
response to nutrient levels
positive regulation of TOR signaling
negative regulation of interleukin-1 production
negative regulation of tumor necrosis factor production
circadian regulation of gene expression
regulation of multicellular organism growth
regulation of gene expression, epigenetic
positive regulation of protein import into nucleus
regulation of circadian rhythm
positive regulation of neuron apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JNK cascade
negative regulation of protein export from nucleus
spindle assembly
random inactivation of X chromosome
neural precursor cell proliferation
establishment of skin barrier
cellular response to mechanical stimulus
cellular response to parathyroid hormone stimulus
cellular response to fluid shear stress
response to dexamethasone
positive regulation of cold-induced thermogenesis
DNA repair-dependent chromatin remodeling
positive regulation of ferroptosis
cornified envelope assembly
positive regulation of type B pancreatic cell apoptotic process
negative regulation of cardiac muscle cell differentiation
chromatin organization
negative regulation of apoptotic process
negative regulation of transcription initiation from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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