|ChIP assay (ChIP)||1-3 ul|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||2.0 µg/ml|
|Western Blot (WB)||See 2 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Dog, Hamster, Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C D(415) G D H D N D K E S D V E I(428) of human HDAC3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-862 detects histone deacetylase 3 (HDAC3) from canine, hamster, human and rat tissues and cells.
PA1-862 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~48 kDa protein representing HDAC3 from HeLa cell lysate.
The PA1-862 immunogen is a synthetic peptide corresponding to residues C D(415) G D H D N D K E S D V E I(428) of human HDAC3. This immunizing peptide (Cat. # PEP-071) is available for use in neutralization and control experiments.
Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p.
HDAC3 is a class I histone deacetylase containing 428 amino acid residues. HDAC3 has been shown to interact directly with the transcription factor YY1 and is expressed in several different cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HD3; Histone Deacetylase 3; histone deacetylase 3-like protein; RPD3-2; SMAP45
Gene Aliases: HD3; HDAC3; RPD3; RPD3-2
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