Immunofluorescent analysis of HDAC3 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a HDAC3 polyclonal antibody (Product # PA1-862) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). HDAC3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
|Tested species reactivity||Dog, Hamster, Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C D(415) G D H D N D K E S D V E I(428) of human HDAC3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1-3 ul|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||2.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-862 detects histone deacetylase 3 (HDAC3) from canine, hamster, human and rat tissues and cells.
PA1-862 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~48 kDa protein representing HDAC3 from HeLa cell lysate.
The PA1-862 immunogen is a synthetic peptide corresponding to residues C D(415) G D H D N D K E S D V E I(428) of human HDAC3. This immunizing peptide (Cat. # PEP-071) is available for use in neutralization and control experiments.
Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p.
HDAC3 is a class I histone deacetylase containing 428 amino acid residues. HDAC3 has been shown to interact directly with the transcription factor YY1 and is expressed in several different cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Regulated clearance of histone deacetylase 3 protects independent formation of nuclear receptor corepressor complexes.
PA1-862 was used in western blot to study the role of regulated HDAC3 turnover in the maintenance of independent complexes of HDAC3 with N-CoR and SMRT
|Guo C,Gow CH,Li Y,Gardner A,Khan S,Zhang J||The Journal of biological chemistry (287:12111)||2012|
The transcriptional repressor Sp3 is associated with CK2-phosphorylated histone deacetylase 2.
PA1-862 was used in western blot to investigate the association between the transcriptional repressor Sp3 and CK2-phosphorylated histone deacetylase 2.
|Sun JM,Chen HY,Moniwa M,Litchfield DW,Seto E,Davie JR||The Journal of biological chemistry (277:35783)||2002|
Differential display cloning of a novel human histone deacetylase (HDAC3) cDNA from PHA-activated immune cells.
PA1-862 was used in immunoprecipitation to isolate and clone the HDAC3 gene and to investigate the regulation of its expression
|Dangond F,Hafler DA,Tong JK,Randall J,Kojima R,Utku N,Gullans SR||Biochemical and biophysical research communications (242:648)||1998|