Invitrogen

HDAC3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (38) HDAC3 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

HDAC3 Antibody in Immunocytochemistry (ICC/IF) — HDAC3 Polyclonal Antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 Polyclonal Antibody [C3], C-term (Product # PA5-29026) diluted at 1:500. Blue: Hoechst 33342 staining.

HDAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HDAC3 (Product # PA5-29026) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

HDAC3 Antibody in Western Blot (WB) — Western Blot using HDAC3 Polyclonal Antibody (Product # PA5-29026). Sample (30 µg whole cell lysate). A: 293T. B: A431. C: H1299. D: HeLa S3. E: HepG2. F: MOLT4. G: Raji. 10% SDS PAGE. HDAC3 Polyclonal Antibody (Product # PA5-29026) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

HDAC3 Antibody in Immunoprecipitation (IP) — HDAC3 antibody immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 1,000 µg 293T whole cell lysate/extract A. 50 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 µg of HDAC3 antibody (Product # PA5-29026) 10% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 antibody (Product # PA5-29026) diluted at 1:1,000.

HDAC3 Antibody in ChIP Assay (ChIP) — HDAC3 antibody immunoprecipitates HDAC3 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract. A: 5 µg preimmune rabbit IgG. B: 5 µg of HDAC3 antibody (Product # PA5-29026). The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

HDAC3 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. 293T cells were transfected with HDAC3 shRNA and decrease in signal intensity was observed in western blot application using HDAC3 polyclonal antibody (Product # PA5-29026).

Product Details

PA5-29026

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

ChIP assay (ChIP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 361 and 428 of Human HDAC3

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.66 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2546502

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, U87-MG, SK-N-SH, IMR32, SK-N-AS, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, FaDu.

Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transcriptomic and genomic studies classify NKL54 as a histone deacetylase inhibitor with indirect influence on MEF2-dependent transcription.

Nucleic acids research

Minisini M,Di Giorgio E,Kerschbamer E,Dalla E,Faggiani M,Franforte E,Meyer-Almes FJ,Ragno R,Antonini L,Mai A,Fiorentino F,Rotili D,Chinellato M,Perin S,Cendron L,Weichenberger CX,Angelini A,Brancolini C

PA5-29026 was used in Western Blot to identify that NKL54 supports MEF2-dependent transcription through several actions, including potentiation of chromatin binding.

Mon Mar 21 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: HD3; HDAC3 splicing HDAC3alpha; HDAC3 splicing HDAC3beta; HDAC3 splicing HDAC3delta; HDAC3 splicing HDAC3gamma; Histone deacetylase 3; Protein deacetylase HDAC3; Protein deacylase HDAC3; RPD3-2; SMAP45

Gene Aliases: AW537363; HD3; HDAC3; RPD3; RPD3-2

UniProt ID: (Human) O15379, (Mouse) O88895, (Rat) Q6P6W3

Entrez Gene ID: (Human) 8841, (Mouse) 15183, (Rat) 84578

Function(s)

Process(es)