Invitrogen

HDAC3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

View all (24) HDAC3 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

HDAC3 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HDAC3 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a HDAC3 polyclonal antibody (Product # PA5-29026) diluted at 1:500. Blue: Hoechst 33342 staining.

HDAC3 Antibody in Immunocytochemistry (ICC) — Immunocytochemistry-Immunofluorescence analysis of HDAC3 was performed in SK-N-SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 Polyclonal Antibody (Product # PA5-29026) diluted at 1:400. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

HDAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HDAC3 in paraffin-embedded SW480 xenograft using a HDAC3 polyclonal antibody (Product # PA5-29026) at a dilution of 1:500.

HDAC3 Antibody in Western Blot (WB) — Western Blot analysis of HDAC3 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a HDAC3 Polyclonal Antibody (Product # PA5-29026) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

HDAC3 Antibody in Immunoprecipitation (IP) — PA5-29026 immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 50 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 µg of HDAC3 antibody (Product # PA5-29026) on 10% SDS-PAGE.

HDAC3 Antibody in ChIP assay (ChIP) — PA5-29026 antibody immunoprecipitates HDAC3 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract with 5 µg preimmune rabbit IgG (A) or 5 µg of HDAC3 antibody (Product # PA5-29026) (B). The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

HDAC3 Antibody in Knockdown — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. 293T cells were transfected with HDAC3 shRNA and decrease in signal intensity was observed in western blot application using HDAC3 polyclonal antibody (Product # PA5-29026). {KD}

Product Details

Applications

Tested Dilution

Publications

ChIP assay (ChIP)

Assay-dependent

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

1:100-1:500

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 361 and 428 of Human HDAC3

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2546502

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, U87-MG, SK-N-SH, IMR32, SK-N-AS, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, *FaDu.

Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Cite this antibody

Bioinformatics

Protein Aliases: HD3; HDAC3 splicing HDAC3alpha; HDAC3 splicing HDAC3beta; HDAC3 splicing HDAC3delta; HDAC3 splicing HDAC3gamma; Histone deacetylase 3; RPD3-2; SMAP45

Gene Aliases: AW537363; HD3; HDAC3; RPD3; RPD3-2

UniProt ID: (Human) O15379, (Rat) Q6P6W3

Entrez Gene ID: (Human) 8841, (Mouse) 15183, (Rat) 84578

Molecular Function: deacetylase hydrolase nucleic acid binding oxidoreductase reductase