Invitrogen

HDAC3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details

View all (19) HDAC3 antibodies

Datasheet

Datasheet

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HDAC3 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HDAC3 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a HDAC3 polyclonal antibody (Product # PA5-29026) diluted at 1:500. Blue: Hoechst 33342 staining.

HDAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HDAC3 in paraffin-embedded SW480 xenograft using a HDAC3 polyclonal antibody (Product # PA5-29026) at a dilution of 1:500.

HDAC3 Antibody in Western Blot (WB) — Western blot analysis of HDAC3 using 30 µg of A) 293T (B) A431 (C) H1299 (D) HeLa S3 (E) HepG2 (F) MOLT4 and G) Raji lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a HDAC3 polyclonal antibody (Product # PA5-29026) at a dilution of 1:1000.

HDAC3 Antibody in Immunoprecipitation (IP) — PA5-29026 immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 50 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 µg of HDAC3 antibody (Product # PA5-29026) on 10% SDS-PAGE.

HDAC3 Antibody in ChIP assay (ChIP) — PA5-29026 antibody immunoprecipitates HDAC3 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract with 5 µg preimmune rabbit IgG (A) or 5 µg of HDAC3 antibody (Product # PA5-29026) (B). The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

HDAC3 Antibody in KnockDown — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. 293T cells were transfected with HDAC3 shRNA and decrease in signal intensity was observed in western blot application using HDAC3 polyclonal antibody (Product # PA529026). {KD}

Product Details

Tested Applications

Dilution

ChIP assay (ChIP)

Assay-dependent

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

1:1000

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 361 and 428 of Human HDAC3

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2546502

Product Specific Information

PA5-29026 targets HDAC3 in IF, IHC (P), and WB applications and shows reactivity with Human samples.

The PA5-29026 immunogen is synthetic peptide corresponding to a region within amino acids 361 and 428 of Human HDAC3.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.