Invitrogen

HDAC3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (32) HDAC3 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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HDAC3 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of HDAC3 was performed in SK-N-SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 Polyclonal Antibody (Product # PA5-29026) diluted at 1:400. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

HDAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HDAC3 in paraffin-embedded SW480 xenograft using a HDAC3 polyclonal antibody (Product # PA5-29026) at a dilution of 1:500.

HDAC3 Antibody in Western Blot (WB) — Western Blot analysis of HDAC3 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a HDAC3 Polyclonal Antibody (Product # PA5-29026) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

HDAC3 Antibody in Immunoprecipitation (IP) — PA5-29026 immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 50 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 µg of HDAC3 antibody (Product # PA5-29026) on 10% SDS-PAGE.

HDAC3 Antibody in ChIP assay (ChIP) — PA5-29026 antibody immunoprecipitates HDAC3 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract with 5 µg preimmune rabbit IgG (A) or 5 µg of HDAC3 antibody (Product # PA5-29026) (B). The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

HDAC3 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. 293T cells were transfected with HDAC3 shRNA and decrease in signal intensity was observed in western blot application using HDAC3 polyclonal antibody (Product # PA5-29026). {KD}

Product Details

PA5-29026

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:100-1:500	-
ChIP assay (ChIP)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to a region within amino acids 361 and 428 of Human HDAC3 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2546502

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, U87-MG, SK-N-SH, IMR32, SK-N-AS, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, FaDu.

Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HD3; HDAC3 splicing HDAC3alpha; HDAC3 splicing HDAC3beta; HDAC3 splicing HDAC3delta; HDAC3 splicing HDAC3gamma; Histone deacetylase 3; RPD3-2; SMAP45

Gene Aliases: AW537363; HD3; HDAC3; RPD3; RPD3-2

UniProt ID: (Human) O15379, (Rat) Q6P6W3

Entrez Gene ID: (Human) 8841, (Mouse) 15183, (Rat) 84578

Function(s)