Invitrogen

HDAC3 Polyclonal Antibody

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HDAC3 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of HDAC3 in 4% paraformaldehyde-fixed HeLa cells using HDAC3 polyclonal antibody (Product # PA5-85378) at a dilution of 1:1000. Sample was then incubated with alpha Tubulin and Hoechst secondary antibody.

HDAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of HDAC3 in paraffin-embedded human ovarian cancer tissue using HDAC3 polyclonal antibody (Product # PA5-85378) at a dilution of 1:250.

HDAC3 Antibody in Western Blot (WB) — Western blot analysis of HDAC3 in non-transfected (–) and transfected (+) 293T whole cell extracts using HDAC3 polyclonal antibody (Product # PA5-85378) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.

HDAC3 Antibody in Immunoprecipitation (IP) — Immunoprecipitation analysis of HDAC3 in 293T whole cell lysate, A) 293T whole cell lysate, B) control of preimmune rabbit IgG, C) immunoprecipitation of HDAC3 protein by of HDAC3 antibody with HDAC3 polyclonal antibody (Product # PA5-85378) using A) 40 µg, B and C) 2 µg of sample at a dilution of 1:1000. Sample was then incubated with anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

HDAC3 Antibody in ChIP assay (ChIP) — ChIP (chromatin immunoprecipitation) analysis of HDAC3 in MCF-7 chromatin extract with HDAC3 polyclonal antibody (Product # PA5-85378) using 5 µg of sample.

Product Details

PA5-85378

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:100-1:500	-
ChIP assay (ChIP)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the center region of human HDAC3.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.32 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% Proclin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2792520

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (89%), Pig (100%), Chicken (95%), Rhesus Monkey (100%), Bovine (100%).

Positive Control: 293T, A431, HeLa, HepG2, U87-MG, SK-N-SH, IMR32, SK-N-AS, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, NB2a.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HD3; HDAC3 splicing HDAC3alpha; HDAC3 splicing HDAC3beta; HDAC3 splicing HDAC3delta; HDAC3 splicing HDAC3gamma; Histone deacetylase 3; RPD3-2; SMAP45

Gene Aliases: AW537363; HD3; HDAC3; RPD3; RPD3-2

UniProt ID: (Human) O15379, (Rat) Q6P6W3

Entrez Gene ID: (Human) 8841, (Mouse) 15183, (Rat) 84578

Function(s)