Invitrogen

HDAC3 Polyclonal Antibody

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HDAC3 Antibody in Immunofluorescence (IF) — Immunocytochemistry analysis of HDAC3 in 4% paraformaldehyde-fixed HeLa cells using HDAC3 polyclonal antibody (Product # PA5-85378) at a dilution of 1:1000. Sample was then incubated with alpha Tubulin and Hoechst secondary antibody.

HDAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of HDAC3 in paraffin-embedded human ovarian cancer tissue using HDAC3 polyclonal antibody (Product # PA5-85378) at a dilution of 1:250.

HDAC3 Antibody in Western Blot (WB) — Western blot analysis of HDAC3 in non-transfected (–) and transfected (+) 293T whole cell extracts using HDAC3 polyclonal antibody (Product # PA5-85378) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.

HDAC3 Antibody in Immunoprecipitation (IP) — Immunoprecipitation analysis of HDAC3 in 293T whole cell lysate, A) 293T whole cell lysate, B) control of preimmune rabbit IgG, C) immunoprecipitation of HDAC3 protein by of HDAC3 antibody with HDAC3 polyclonal antibody (Product # PA5-85378) using A) 40 µg, B and C) 2 µg of sample at a dilution of 1:1000. Sample was then incubated with anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

HDAC3 Antibody in ChIP assay (ChIP) — ChIP (chromatin immunoprecipitation) analysis of HDAC3 in MCF-7 chromatin extract with HDAC3 polyclonal antibody (Product # PA5-85378) using 5 µg of sample.

Product Details

Tested Applications

Dilution

ChIP assay (ChIP)

Assay-dependent

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

1:100-1:500

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human HDAC3.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.32 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Keep as concentrated solution.

Positive Control: 293T, A431, HeLa, HepG2, U87-MG, SK-N-SH, IMR32, SK-N-AS, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, NB2a.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HD3; HD3 antibody; HDAC3 antibody; HDAC3 splicing HDAC3alpha; HDAC3 splicing HDAC3beta; HDAC3 splicing HDAC3delta; HDAC3 splicing HDAC3gamma; Histone deacetylase 3; histone deacetylase 3 antibody; RPD3 antibody; RPD3-2; RPD3-2 antibody; SMAP45; SMAP45 antibody

Gene Aliases: AW537363; HD3; HDAC3; RPD3; RPD3-2

UniProt ID: (Human) O15379, (Rat) Q6P6W3

Entrez Gene ID: (Human) 8841, (Mouse) 15183, (Rat) 84578

Molecular Function: deacetylase hydrolase nucleic acid binding oxidoreductase reductase