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Invitrogen
HDAC4 Polyclonal Antibody
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FIGURE: 1 / 3
HDAC4 Antibody (PA1-863) in IHC (P)
Immunohistochemistry was performed on normal biopsies of deparaffinized human skin tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Rabbit Polyclonal Antib... View More
Product Details
PA1-863
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues M(1)SSQSHPDGLSGRDQPVE(18) of human HDAC4
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-863 detects histone deacetylase 4 (HDAC4) from human cells.
PA1-863 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~140 kDa protein representing HDAC4 from HeLa cell lysate. An ~110 kDa protein is also detected with PA1-863 which could correspond to protein degradation product.
PA1-863 immunogen is a synthetic peptide corresponding to residues M(1)SSQSHPDGLSGRDQPVE(18) of human HDAC4.
PA1-863 can be used with blocking peptide PEP-141 and blocking peptide PEP-095.
Target Information
Histone deacetylase 4 (HDAC4) is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is also involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. It is also involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Mutations to the HDAC4 gene have been found in Brachydactyly-mental retardation syndrome (BDMR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp779K1053; HD4; HD7; HD7b; HD9; HDAC; HDAC4; HDAC7; HDAC7B; HDAC9; HDAC9B; HDAC9FL; HDRP; Histone deacetylase 4; histone deacetylase A; KIAA0744; MITR; unnamed protein product
Gene Aliases: AHO3; BDMR; HA6116; HD4; HDAC-4; HDAC-A; HDAC4; HDACA; KIAA0288; NEDCHF; NEDCHID
UniProt ID: (Human) P56524
Entrez Gene ID: (Human) 9759
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
histone deacetylase activity
protein binding
zinc ion binding
hydrolase activity
SUMO transferase activity
potassium ion binding
protein deacetylase activity
histone binding
identical protein binding
histone deacetylase binding
metal ion binding
binding, bridging
RNA polymerase II sequence-specific DNA binding transcription factor binding
DNA-binding transcription factor binding
histone deacetylase activity, hydrolytic mechanism
negative regulation of transcription from RNA polymerase II promoter
chromatin organization
chromatin remodeling
protein deacetylation
inflammatory response
nervous system development
positive regulation of cell proliferation
negative regulation of macromolecule biosynthetic process
negative regulation of myotube differentiation
negative regulation of transcription by competitive promoter binding
response to denervation involved in regulation of muscle adaptation
cardiac muscle hypertrophy in response to stress
protein sumoylation
B cell differentiation
positive regulation of protein sumoylation
regulation of gene expression, epigenetic
B cell activation
negative regulation of gene expression, epigenetic
negative regulation of glycolytic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
type I interferon signaling pathway
response to interleukin-1
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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